@article{fdi:010070953,
  title = {{E}ffect of amino acid substitutions within the {V}3 region of {HIV}-1 {CRF}01_{AE} on interaction with {CCR}5-coreceptor},
  author = {{H}ongjaisee, {S}. and {B}raibant, {M}. and {B}arin, {F}. and {N}go-{G}iang-{H}uong, {N}icole and {S}irirungsi, {W}. and {S}amleerat, {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}pecific amino acids within the {V}3 loop of {HIV}-1 {CRF}01_{AE} envelope glycoprotein that are involved in the interaction with {CCR}5/{CXCR}4 coreceptors, are not well characterized. {W}e generated {V}3 mutants using polymerase chain reaction ({PCR})-based site-directed mutagenesis of {HIV}-1 {CRF}01_{AE} {R}5-env plasmids at specific positions. {M}utant viruses were produced by env-pseudotyped virus assay, tested for coreceptor usage using {U}373.{R}5 and {U}373.{X}4 cells, and viral entry was assessed with luciferase activity measurement. {A}ll viruses, harboring either single or double mutations, used the {CCR}5 coreceptor. {H}owever, those containing a single substitution at positions 7, 11, 18, and 32 and those with mutations at positions 5/32 and 18/32 had reduced infectivity. {O}nly virus with arginine substitution at position 11 seemed to be involved in {CXCR}4 coreceptor usage. {O}ur results suggest that some {V}3 positions may be necessary for the binding to coreceptor, but not for the switch of coreceptor usage.},
  keywords = {{HIV}-1 ; {CRF}01_{AE} ; {V}3 ; coreceptor usage ; {CCR}5},
  booktitle = {},
  journal = {{A}ids {R}esearch and {H}uman {R}etroviruses},
  volume = {33},
  numero = {9},
  pages = {946--951},
  ISSN = {0889-2229},
  year = {2017},
  DOI = {10.1089/aid.2017.0044},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070953},
}