@article{fdi:010070932,
  title = {{D}o the venous blood samples replicate malaria parasite densities found in capillary blood ? {A} field study performed in naturally-infected asymptomatic children in {C}ameroon},
  author = {{S}andeu, {M}. {M}. and {B}ayibeki, {A}. {N}. and {T}chioffo, {M}. {T}. and {A}bate, {L}uc and {G}imonneau, {G}. and {A}wono-{A}mbene, {P}. {H}. and {N}sango, {S}. {E}. and {D}iallo, {D}. and {B}erry, {A}. and {T}exier, {G}. and {M}orlais, {I}sabelle},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he measure of new drug-or vaccine-based approaches for malaria control is based on direct membrane feeding assays ({DMFA}s) where gametocyte-infected blood samples are offered to mosquitoes through an artificial feeder system. {G}ametocyte donors are identified by the microscopic detection and quantification of malaria blood stages on blood films prepared using either capillary or venous blood. {H}owever, parasites are known to sequester in the microvasculature and this phenomenon may alter accurate detection of parasites in blood films. {T}he blood source may then impact the success of mosquito feeding experiments and investigations are needed for the implementation of {DMFA}s under natural conditions. {M}ethods: {T}hick blood smears were prepared from blood obtained from asymptomatic children attending primary schools in the vicinity of {M}fou ({C}ameroon) over four transmission seasons. {P}arasite densities were determined microscopically from capillary and venous blood for 137 naturally-infected gametocyte carriers. {T}he effect of the blood source on gametocyte and asexual stage densities was then assessed by fitting cumulative link mixed models ({CLMM}). {DMFA}s were performed to compare the infectiousness of gametocytes from the different blood sources to mosquitoes. {R}esults: {P}revalence of {P}lasmodium falciparum asexual stages among asymptomatic children aged from 4 to 15 years was 51.8% (2116/4087). {T}he overall prevalence of {P}. falciparum gametocyte carriage was 8.9% and varied from one school to another. {N}o difference in the density of gametocyte and asexual stages was found between capillary and venous blood. {A}ttempts to perform {DMFA}s with capillary blood failed. {C}onclusions: {P}lasmodium falciparum malaria parasite densities do not differ between capillary and venous blood in asymptomatic subjects for both gametocyte and trophozoite stages. {T}his finding suggests that the blood source should not interfere with transmission efficiency in {DMFA}s.},
  keywords = {{P}lasmodium falciparum ; {M}embrane feeding ; {B}lood source ; {M}alaria ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {16},
  numero = {},
  pages = {art. 345 [9 p.]},
  ISSN = {1475-2875},
  year = {2017},
  DOI = {10.1186/s12936-017-1978-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070932},
}