@article{fdi:010070920,
  title = {{G}enomic, transcriptomic, and proteomic approaches towards understanding the molecular mechanisms of salt tolerance in {F}rankia strains isolated from {C}asuarina trees},
  author = {{O}shone, {R}. and {N}gom, {M}. and {C}hu, {F}. {X}. and {M}ansour, {S}. and {S}y, {M}. {O}. and {C}hampion, {A}ntony and {T}isa, {L}. {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {S}oil salinization is a worldwide problem that is intensifying because of the effects of climate change. {A}n effective method for the reclamation of salt-affected soils involves initiating plant succession using fast growing, nitrogen fixing actinorhizal trees such as the {C}asuarina. {T}he salt tolerance of {C}asuarina is enhanced by the nitrogen-fixing symbiosis that they form with the actinobacterium {F}rankia. {I}dentification and molecular characterization of salt-tolerant {C}asuarina species and associated {F}rankia is imperative for the successful utilization of {C}asuarina trees in saline soil reclamation efforts. {I}n this study, salt-tolerant and salt-sensitive {C}asuarina associated {F}rankia strains were identified and comparative genomics, transcriptome profiling, and proteomics were employed to elucidate the molecular mechanisms of salt and osmotic stress tolerance. {R}esults: {S}alt-tolerant {F}rankia strains ({C}c{I}6 and {A}llo2) that could withstand up to 1000 m{M} {N}a{C}l and a salt-sensitive {F}rankia strain ({C}c{I}3) which could withstand only up to 475 m{M} {N}a{C}l were identified. {T}he remaining isolates had intermediate levels of salt tolerance with {MIC} values ranging from 650 m{M} to 750 m{M}. {C}omparative genomic analysis showed that all of the {F}rankia isolates from {C}asuarina belonged to the same species ({F}rankia casuarinae). {P}angenome analysis revealed a high abundance of singletons among all {C}asuarina isolates. {T}he two salt-tolerant strains contained 153 shared single copy genes (most of which code for hypothetical proteins) that were not found in the salt-sensitive({C}c{I}3) and moderately salttolerant ({C}e{D}) strains. {RNA}-seq analysis of one of the two salt-tolerant strains ({F}rankia sp. strain {C}c{I}6) revealed hundreds of genes differentially expressed under salt and/or osmotic stress. {A}mong the 153 genes, 7 and 7 were responsive to salt and osmotic stress, respectively. {P}roteomic profiling confirmed the transcriptome results and identified 19 and 8 salt and/or osmotic stress-responsive proteins in the salt-tolerant ({C}c{I}6) and the salt-sensitive ({C}c{I}3) strains, respectively. {C}onclusion: {G}enetic differences between salt-tolerant and salt-sensitive {F}rankia strains isolated from {C}asuarina were identified. {T}ranscriptome and proteome profiling of a salt-tolerant strain was used to determine molecular differences correlated with differential salt-tolerance and several candidate genes were identified. {M}echanisms involving transcriptional and translational regulation, cell envelop remodeling, and previously uncharacterized proteins appear to be important for salt tolerance. {P}hysiological and mutational analyses will further shed light on the molecular mechanism of salt tolerance in {C}asuarina associated {F}rankia isolates.},
  keywords = {{A}ctinobacteria ; {A}ctinorhizal symbiosis ; {C}omparative genomics ; {S}alt stress ; {S}alt tolerance ; {T}ranscriptomics},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {18},
  numero = {},
  pages = {art. 633 [21 p.]},
  ISSN = {1471-2164},
  year = {2017},
  DOI = {10.1186/s12864-017-4056-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070920},
}