@article{fdi:010070854,
  title = {{S}tatus of insecticide resistance in {A}nopheles mosquitoes in {U}bon {R}atchathani province, {N}ortheastern {T}hailand},
  author = {{S}umarnrote, {A}. and {O}vergaard, {H}. {J}. and {M}arasri, {N}. and {F}ustec, {B}{\'e}n{\'e}dicte and {T}hanispong, {K}. and {C}hareonviriyaphap, {T}. and {C}orbel, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}alaria is common in hilly, forested areas along national borders in {S}outheast {A}sia. {I}nsecticide resistance in malaria vectors has been detected in a few countries in the {G}reater {M}ekong sub-region ({GMS}), representing a threat to malaria control and prevention. {T}his study aims to determine the insecticide resistance status of {A}nopheles mosquitoes in {U}bon {R}atchathani province, northeastern {T}hailand, where increasing number of malaria cases were reported recently. {M}ethods: {M}osquitoes were collected in 2013-2015 using human landing and cattle bait collections in six sites during both the rainy and dry seasons. {M}osquitoes were first morphologically identified to species and their susceptibility status to deltamethrin (0.05%), permethrin (0.75%) and {DDT} (4%) investigated, according to {WHO} guidelines. {B}ioassays with the synergists {PBO} and {DEF} were carried out to address the role of detoxifying enzymes in insecticide resistance. {DNA} sequencing of a fragment of the voltage-gated sodium channel gene was carried out to detect knock-down resistance (kdr) substitutions at position 1014 in resistant species. {R}esults: {D}ue to low vector abundance, complete bioassays (n >= 100 mosquitoes) were only achieved for {A}nopheles hyrcanus s.l., which was resistant to all insecticides tested (mortality ranged from 45 to 87%). {S}uspected resistance to {DDT} was found in {A}nopheles barbirostris s.l. (mortality 69%), but it was susceptible to deltamethrin (mortality 97-100%) and permethrin (mortality 100%). {A}lthough insufficient number of primary vectors were collected, results showed that {A}nopheles dirus s.l. and {A}nopheles maculatus s.l. were susceptible to deltamethrin (mortality 100%). {A}nopheles nivipes and {A}nopheles philippinensis were susceptible to all three insecticides. {PBO} significantly increased mortality to deltamethrin and permethrin in pyrethroid-resistant {A}n. hyrcanus s.l. {N}one of the sequenced specimens presented the {L}1014{F} or {L}1014{S} mutation. {D}iscussion: {T}his study shows that insecticide resistance is present in potential malaria vectors in northeastern {T}hailand. {T}he absence of kdr mutations in all {A}nopheles species tested suggests that metabolic resistance is the main mechanism of pyrethroid resistance. {T}his study provides new findings about insecticide susceptibility status of potential malaria vectors in northeastern {T}hailand that are deemed important to guide malaria vector control.},
  keywords = {{M}alaria ; {V}ectors ; {A}nopheles ; {P}yrethroids ; {DDT} ; {R}esistance ; {S}ynergists ; kdr ; {THAILANDE}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {16},
  numero = {},
  pages = {art. 299 [13 p.]},
  ISSN = {1475-2875},
  year = {2017},
  DOI = {10.1186/s12936-017-1948-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070854},
}