%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Diallo-Diagne, N.H. %A Assigbetsé, Komi %A Sall, S. %A Masse, Dominique %A Bonzi, M. %A Ndoye, I. %A Chotte, Jean-Luc %T Response of soil microbial properties to long-term application of organic and inorganic amendments in a tropical soil (Saria, Burkina Faso) %D 2016 %L fdi:010070579 %G ENG %J Open Journal of Soil Science %@ 2162-5360 %K FERTILITE DU SOL ; MICROBIOLOGIE DU SOL ; MATIERE ORGANIQUE ; ENGRAIS MINERAL ; BIOMASSE %K BURKINA FASO ; ZONE SOUDANOSAHELIENNE %N 6 %P 21-33 %R 10.4236/ojss.2016.62003 %U https://www.documentation.ird.fr/hor/fdi:010070579 %> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-09/010070579.pdf %V 6 %W Horizon (IRD) %X Soil microbial biomass carbon (MBC), β-glucosidase, acid phosphatase and fluorescein diacetate (FDA) activities and bacterial community structure were assessed in a long-term (26 years) experiment, at physiological stages of sorghum growth, comparing different management methods for organic (manure, straw residues) and inorganic (urea) amendments at the INERA field station in Saria (Burkina Faso). Annual application of manure led to the highest soil microbial biomass and enzyme activities. Investigations indicated that only microbial biomass and β-glucosidase activities were affected during the cropping season. Phosphatase and FDA enzyme activities did not depend on the crop development stages. The application of N fertilizer modified phosphatase and FDA enzyme activities, the activities being higher in soils amended with N fertilizer. The bacterial community structure was analyzed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the eubacterial 16S rRNA gene. Cluster analysis of PCR-DGGE patterns showed two major clusters, the first containing the mineral fertilization and straw treatments and the second, the straw + urea, manure and manure + urea treatments. Sorghum grain yields were the highest for manure treatments. In this long-term experiment, applying straw did not produce a better grain yield than that obtained in the un-amended plot. %$ 068EROSOL ; 076AGRO