@article{fdi:010070406,
  title = {{R}ole of auxin during intercellular infection of {D}iscaria trinervis by {F}rankia},
  author = {{I}manishi, {L}. and {P}errine-{W}alker, {F}.{M}. and {N}dour, {A}. and {V}ayssi{\`e}res, {A}. and {C}onejero, {G}. and {L}ucas, {M}ika{\¨e}l and {C}hampion, {A}ntony and {L}aplaze, {L}aurent and {W}all, {L}. and {S}vistoonoff, {S}ergio},
  editor = {},
  language = {{ENG}},
  abstract = {{N}itrogen-fixing nodules induced by {F}rankia in the actinorhizal plant {D}iscaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. {H}ere, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant {C}asuarina glauca. {I}mmunolocalisation experiments showed that auxin accumulated in {F}rankia-infected cells in both systems. {W}e then characterized the expression of auxin transporters in {D}. trinervis nodules. {N}o activation of the heterologous {C}g{AUX}1 promoter was detected in infected cells in {D}. trinervis. {T}hese results were confirmed with the endogenous {D}. trinervis gene, {D}t{AUX}1. {H}owever, {D}t{AUX}1 was expressed in the nodule meristem. {C}onsistently, transgenic {D}. trinervis plants containing the auxin response marker {DR}5:{VENUS} showed expression of the reporter gene in the meristem. {I}mmunolocalisation experiments using an antibody against the auxin efflux carrier {PIN}1, revealed the presence of this transporter in the plasma membrane of infected cells. {F}inally, we used in silico cellular models to analyse auxin fluxes in {D}. trinervis nodules. {O}ur results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.},
  keywords = {},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {5},
  numero = {},
  pages = {art. no 399 [9  en ligne]},
  ISSN = {1664-462{X}},
  year = {2014},
  DOI = {10.3389/fpls.2014.00399},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070406},
}