@article{fdi:010070390,
  title = {{G}enetic analyses of {HIV} env associated with uveitis in antiretroviral-naive individuals},
  author = {{W}illiams-{W}ietzikoski, {C}. {A}. and {S}o, {I}. {D}. and {B}ull, {M}. {E}. and {S}amleerat, {T}. and {P}athanapitoon, {K}. and {K}unavisarut, {P}. and {K}ongyai, {N}. and {N}go-{G}iang-{H}uong, {N}icole and {F}renkel, {L}. {M}. and {S}irirungsi, {W}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {T}o analyze and compare {HIV}-1 env sequences from the eye to those from the blood of individuals with uveitis attributed to {HIV} with the goal of gaining insight into the pathogenesis of {HIV}-associated eye disease. {D}esign: {A} prospective case series of five {HIV}-infected antiretroviral-naive individuals with uveitis negative for other pathogens. {M}ethods: {RNA} from blood plasma and ocular aqueous humor was reverse transcribed using random hexamers. {HIV} env {C}2-{V}5 ({HXB}2: 6990-7668) sequences were generated by single-genome amplification using nested polymerase chain reaction followed by bidirectional {S}anger sequencing. {S}equence analyses by {G}eneious, {G}eno2{P}heno, {N}-{GLYCOSITE}, {DIVEIN}, and {H}y{P}hy evaluated relationships between {HIV} in plasma and aqueous humor. {R}esults: {A} median of 20 (range: 13-22) plasma and 15 (range: 9-18) aqueous humor sequences were generated from each individual. {T}he frequencies of sequences with predicted-{N}-linked-glycosylation sites and {C}-{X}-{C} chemokine receptor type 4 were comparable in aqueous humor and plasma of all five patients. {A}queous humor sequences had lower median genetic diversity compared with plasma across all patients, but similar divergence, in four of five patients. {A}queous humor {HIV} sequences were compartmentalized from plasma across subjects by {C}ritchlow correlation coefficient, {S}latkin and {M}addison, nearest-neighbor statistic, and {F}ixation index. {C}onclusion: {A}mong antiretroviral-naive individuals with uveitis attributed to {HIV}, the universal compartmentalization and decreased diversity of eye compared with blood sequences suggests time-limited passage of a small subset of variants from each patient's viral population into the eye tissues, followed by limited immune selection despite the inflammatory uveitis.},
  keywords = {aqueous humor ; {C}-{X}-{C} chemokine receptor type 4 and {C}-{C} chemokine ; receptor type 5 coreceptor usage ; {HIV}-1 ; predicted {N}-linked ; glycosylation sites ; single-genome amplification ; uveitis ; virus ; compartmentalization ; {THAILANDE}},
  booktitle = {},
  journal = {{AIDS}},
  volume = {31},
  numero = {13},
  pages = {1825--1830},
  ISSN = {0269-9370},
  year = {2017},
  DOI = {10.1097/qad.0000000000001550},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070390},
}