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Alves G. S. C., Torres L. F., Dechamp E., Breitler J. C., Joët Thierry, Gatineau F., Andrade A. C., Bertrand B., Marraccini P., Etienne H. (2017). Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit. Journal of Experimental Botany, 68 (11), 3017-3031. ISSN 0022-0957

Fichier PDF disponible http://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-08/010070376.pdf

Lien direct chez l'éditeur doi:10.1093/jxb/erx166

Titre
Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit
Année de publication2017
Type de documentArticle référencé dans le Web of Science WOS:000405579100031
AuteursAlves G. S. C., Torres L. F., Dechamp E., Breitler J. C., Joët Thierry, Gatineau F., Andrade A. C., Bertrand B., Marraccini P., Etienne H.
SourceJournal of Experimental Botany, 2017, 68 (11), p. 3017-3031. ISSN 0022-0957
RésuméDespite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and drought-sensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (-2.0 MPa) and low relative humidity treatments. A deletion analysis of their corresponding 5'-upstream regions revealed increased specificity of beta-glucuronidase activity in the polyethylene glycol and low relative humidity treatments, with high expression in leaf mesophyll and guard cells in full-length constructs. RT-qPCR assays also revealed that the HP16 haplotype (specific to clone tolerant to water deficit) had stronger and earlier activity compared with the HP15 and HP17 haplotypes. As most of the cis-regulatory elements involved in ABA-dependent and -independent networks, tissue specificity and light regulation are common to these haplotypes, we propose that their organization, as well as the nucleic acid polymorphisms present outside these boxes, may play a role in modulating activities of DREB1D promoters in guard cells.
Plan de classementSciences du monde végétal [076]
LocalisationFonds IRD [F B010070376]
Identifiant IRDfdi:010070376
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010070376

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