@article{fdi:010070275,
  title = {{C}omparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with {P}lasmodium falciparum field isolates},
  author = {{E}ssone, {J}cbb and {N}'{D}ilimabaka, {N}. and {O}ndzaga, {J}. and {L}ekana-{D}ouki, {J}. {B}. and {M}ba, {D}. {N}. and {D}eloron, {P}hilippe and {M}azier, {D}. and {G}ay, {F}. and {N}douo, {F}. {S}. {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}lasmodium falciparum infection can progress unpredictably to severe forms including respiratory distress and cerebral malaria. {T}he mechanisms underlying the variable natural course of malaria remain elusive. {M}ethods: {T}he cerebral microvascular endothelial cells-{D}3 and lung endothelial cells both from human were cultured separately and challenged with {P}. falciparum field isolates taken directly from malaria patients or 3{D}7 strain (in vitro maintained culture). {T}he capacity of these {P}. falciparum isolates to induce endothelial cell apoptosis via cytoadherence or not was then assessed. {R}esults: {O}verall, 27 {P}. falciparum isolates were collected from patients with uncomplicated malaria (n = 25) or severe malaria (n = 2). {A}bout half the isolates (n = 17) were able to bind brain endothelial cells (12 isolates, 44%) or lung endothelial cells (17 isolates, 63%) or both (12 isolates, 44%). {S}ixteen (59%) of the 27 isolates were apoptogenic for brain and/or lung endothelial cells. {T}he apoptosis stimulus could be cytoadherence, direct cell-cell contact without cytoadherence, or diffusible soluble factors. {W}hile some of the apoptogenic isolates used two stimuli (direct contact with or without cytoadherence, plus soluble factors) to induce apoptosis, others used only one. {A}mong the 16 apoptogenic isolates, eight specifically targeted brain endothelial cells, one lung endothelial cells, and seven both. {C}onclusion: {T}hese results indicate that the brain microvascular cell line was more susceptible to apoptosis triggered by {P}. falciparum than the primary pulmonary endothelial cells and may have relevance to host-parasite interaction.},
  keywords = {{M}alaria ; {P}lasmodium falciparum ; {E}ndothelial cell death ; {GABON}},
  booktitle = {},
  journal = {{BMC} {I}nfectious {D}iseases},
  volume = {17},
  numero = {},
  pages = {art. 454 [10 p.]},
  ISSN = {1471-2334},
  year = {2017},
  DOI = {10.1186/s12879-017-2552-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070275},
}