@article{fdi:010070271,
  title = {{V}itamin {A} affects flatfish development in a thyroid hormone signaling and metamorphic stage dependent manner},
  author = {{F}ernandez, {I}. and {O}rtiz-{D}elgado, {J}. {B}. and {D}arias, {M}aria {J}ose and {H}ontoria, {F}. and {A}ndree, {K}. {B}. and {M}anchado, {M}. and {S}arasquete, {C}. and {G}isbert, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{V}itamin {A} ({VA}) and retinoid derivatives are known morphogens controlling vertebrate development. {D}espite the research effort conducted during the last decade, the precise mechanism of how {VA} induces post-natal bone changes, and particularly those operating through crosstalk with the thyroid hormones ({TH}s) remain to be fully understood. {S}ince effects and mechanisms seem to be dose and time-dependent, flatfish are an interesting study model as they undergo a characteristic process of metamorphosis driven by {TH}s that can be followed by external appearance. {H}ere, we studied the effects of {VA} imbalance that might determine {S}enegalese sole ({S}olea senegalensis) skeletogenetic phenotype through development of thyroid follicles, {TH}s homeostasis and signaling when a dietary {VA} excess was specifically provided during pre-, pro-or post-metamorphic stages using enriched rotifers and {A}rtemia as carriers. {T}he increased {VA} content in enriched live prey was associated to a higher {VA} content in fish at all developmental stages. {D}ietary {VA} content clearly affected thyroid follicle development, {T}3 and {T}4 immunoreactive staining, skeletogenesis and mineralization in a dose and time-dependent fashion. {G}ene expression analysis showed that {VA} levels modified the m{RNA} abundance of {VA}- and {TH}-specific nuclear receptors at specific developmental stages. {P}resent results provide new and key knowledge to better understand how {VA} and {TH} pathways interact at tissue, cellular and nuclear level at different developmental periods in {S}enegalese sole, unveiling how dietary modulation might determine juvenile phenotype and physiology.},
  keywords = {vitamin {A} ; thyroidal follicles ; skeletogenesis ; ossification ; nuclear ; receptors ; flatfish ; {S}enegalese sole ; {S}olea senegalensis},
  booktitle = {},
  journal = {{F}rontiers in {P}hysiology},
  volume = {8},
  numero = {},
  pages = {art. 458 [20 p.]},
  ISSN = {1664-042{X}},
  year = {2017},
  DOI = {10.3389/fphys.2017.00458},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070271},
}