@article{fdi:010070246,
  title = {{A}daptation and optimization of a fluorescence-based assay for in vivo antimalarial drug screening},
  author = {{A}rias, {M}. {H}. and {D}eharo, {E}ric and {V}alentin, {A}. and {G}aravito, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he in vivo efficacy of potential antimalarials is usually evaluated by direct microscopic determination of the parasitaemia of {P}lasmodium-infected mice on {G}iemsa-stained blood smears. {T}his process is time-consuming, requires experienced technicians and is not automatable. {T}herefore, we optimized a {SYBR} {G}reen {I} ({SYBRG} {I}) fluorescence-based assay to fluorometers commonly available in many research laboratories. {T}his technique was originally developed to assess parasitaemia in humans by cytometry. {W}e defined optimal conditions with {P}lasmodium berghei-infected mice, standard lysis buffer ({T}ris, {EDTA}, saponin and {T}riton), whole blood cells and 2 h staining incubation with {SYBRG} {I} 2{X}. {T}he fluorescence background generated by uninfected whole blood cells was low (around 4.6%), and the linearity high (r(2) = 0.96), with parasitaemia ranging from 1.4 to 60%. {T}he {B}land-{A}ltman plot showed a strong correlation between {SYBRG} {I} and {G}iemsa gold standard method; {Z}'-factor was >0.5. {T}hese findings suggest that our fluorescence-based assay is suitable for in vivo antimalarial drug assessment in a malaria murine model. {I}t can help to overcome the human bias found with microscopic techniques.},
  keywords = {{M}alaria ; {P}harmacology ; {P}lasmodium berghei ; {SYBR} {G}reen {I}},
  booktitle = {},
  journal = {{P}arasitology {R}esearch},
  volume = {116},
  numero = {7},
  pages = {1955--1962},
  ISSN = {0932-0113},
  year = {2017},
  DOI = {10.1007/s00436-017-5477-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070246},
}