@article{fdi:010070245,
  title = {{M}olecular investigation and phylogeny of {A}naplasmataceae species infecting domestic animals and ticks in {C}orsica, {F}rance},
  author = {{D}ahmani, {M}. and {D}avoust, {B}. and {T}ahir, {D}. and {R}aoult, {D}. and {F}enollar, {F}. and {M}ediannikov, {O}leg},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackgrounds: {C}orsica is a {F}rench island situated in the {M}editerranean {S}ea. {T}he island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. {T}he family {A}naplasmataceae is a member of the order {R}ickettsiales; it includes the genera {A}naplasma, {E}hrlichia, {N}eorickettsia and {W}olbachia. {A}naplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera {A}naplasma and {E}hrlichia. {T}he aim of this study was to identify and estimate the prevalence of {A}naplasmataceae species infecting domestic animals and ticks in {C}orsica. {M}ethods: {I}n this study, 458 blood samples from sheep, cattle, horses, goats, dogs, and 123 ticks removed from cattle, were collected in {C}orsica. {Q}uantitative real-time {PCR} screening and genetic characterisation of {A}naplasmataceae bacteria were based on the 23{S} r{RNA}, rpo{B} and gro{E}l genes. {R}esults: {T}wo tick species were collected in the present study: {R}hipicephalus bursa (118) and {H}yalomma marginatum marginatum (5). {M}olecular investigation showed that 32.1% (147/458) of blood samples were positive for {A}naplasmataceae infection. {A}naplasma ovis was identified in 42.3% (93/220) of sheep. {A}naplasma marginale was amplified from 100% (12/12) of cattle and two {R}. bursa (2/123). {S}everal potentially new species were also identified: {A}naplasma cf. ovis, "{C}andidatus {A}naplasma corsicanum", "{C}andidatus {A}naplasma mediterraneum" were amplified from 17.3% (38/220) of sheep, and {A}naplasma sp. marginale-like was amplified from 80% (4/5) of goats. {F}inally, one {R}. bursa tick was found to harbour the {DNA} of {E}. canis. {A}ll samples from horses and dogs were negative for {A}naplasmataceae infection. {C}onclusions: {T}o our knowledge, this study is the first epidemiological survey on {A}naplasmataceae species infecting animals and ticks in {C}orsica and contributes toward the identification of current {A}naplasmataceae species circulating in {C}orsica.},
  keywords = {{C}orsica {I}sland ; {A}nimals ; {T}icks ; {A}naplasma ovis ; {A}naplasma marginale ; {A}naplasma sp. ; {E}hrlichia canis ; {FRANCE} ; {CORSE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {10},
  numero = {},
  pages = {art. 302 [12 p.]},
  ISSN = {1756-3305},
  year = {2017},
  DOI = {10.1186/s13071-017-2233-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070245},
}