@article{fdi:010070204,
  title = {{I}mipramine inhibits chikungunya virus replication in human skin fibroblasts through interference with intracellular cholesterol trafficking},
  author = {{W}ichit, {S}ineewanlaya and {H}amel, {R}odolphe and {B}ernard, {E}. and {T}alignani, {L}. and {D}iop, {F}od{\'e} and {F}erraris, {P}auline and {L}i{\'e}geois, {F}lorian and {E}kchariyawat, {P}. and {L}uplertlop, {N}. and {S}urasombatpattana, {P}. and {T}homas, {F}. and {M}erits, {A}. and {C}houmet, {V}. and {R}oques, {P}. and {Y}ssel, {H}. and {B}riant, {L}. and {M}iss{\'e}, {D}oroth{\'e}e},
  editor = {},
  language = {{ENG}},
  abstract = {{C}hikungunya virus ({CHIKV}) is an emerging arbovirus of the {T}ogaviridae family that poses a present worldwide threat to human in the absence of any licensed vaccine or antiviral treatment to control viral infection. {H}ere, we show that compounds interfering with intracellular cholesterol transport have the capacity to inhibit {CHIKV} replication in human skin fibroblasts, a major viral entry site in the human host. {P}retreatment of these cells with the class {II} cationic amphiphilic compound {U}18666{A}, or treatment with the {FDA}-approved antidepressant drug imipramine resulted in a near total inhibition of viral replication and production at the highest concentration used without any cytotoxic effects. {I}mipramine was found to affect both the fusion and replication steps of the viral life cycle. {T}he key contribution of cholesterol availability to the {CHIKV} life cycle was validated further by the use of fibroblasts from {N}iemann-{P}ick type {C} ({NPC}) patients in which the virus was unable to replicate. {I}nterestingly, imipramine also strongly inhibited the replication of several {F}laviviridae family members, including {Z}ika, {W}est {N}ile and {D}engue virus. {T}ogether, these data show that this compound is a potential drug candidate for anti-arboviral treatment.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {7},
  numero = {},
  pages = {art. 3145 [12 ]},
  ISSN = {2045-2322},
  year = {2017},
  DOI = {10.1038/s41598-017-03316-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070204},
}