@article{fdi:010070203,
  title = {{B}urkholderia pseudomallei in a lowland rice paddy : seasonal changes and influence of soil depth and physico-chemical properties},
  author = {{M}anivanh, {L}. and {P}ierret, {A}lain and {R}attanavong, {S}. and {K}ounnavongsa, {O}. and {B}uisson, {Y}. and {E}lliott, {I}. and {M}aeght, {J}ean-{L}uc and {X}ayyathip, {K}. and {S}ilisouk, {J}. and {V}ongsouvath, {M}. and {P}hetsouvanh, {R}. and {N}ewton, {P}. {N}. and {L}acombe, {G}. and {R}ibolzi, {O}livier and {R}ochelle {N}ewall, {E}mma and {D}ance, {D}. {A}. {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}elioidosis, a severe infection with the environmental bacterium {B}urkholderia pseudomallei, is being recognised increasingly frequently. {W}hat determines its uneven distribution within endemic areas is poorly understood. {W}e cultured soil from a rice field in {L}aos for {B}. pseudomallei at different depths on 4 occasions over a 13-month period. {W}e also measured physical and chemical parameters in order to identify associated characteristics. {O}verall, 195 of 653 samples (29.7%) yielded {B}. pseudomallei. {A} higher prevalence of {B}. pseudomallei was found at soil depths greater than the 30 cm currently recommended for {B}. pseudomallei environmental sampling. {B}. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. {O}ur results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 x 25 m) should be sufficient to detect {B}. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. {H}owever, culture of {B}. pseudomallei in environmental samples is difficult and liable to variation. {F}uture studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of {B}. pseudomallei.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {7},
  numero = {},
  pages = {art. 3031 [11 p.]},
  ISSN = {2045-2322},
  year = {2017},
  DOI = {10.1038/s41598-017-02946-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070203},
}