@article{fdi:010070101,
  title = {{A}n integrated overview of the midgut bacterial flora composition of {P}hlebotomus perniciosus, a vector of zoonotic visceral leishmaniasis in the {W}estern {M}editerranean {B}asin},
  author = {{F}raihi, {W}. and {F}ares, {W}. and {P}errin, {P}. and {D}orkeld, {F}. and {S}ereno, {D}enis and {B}arhoumi, {W}. and {S}bissi, {I}. and {C}herni, {S}. and {C}helbi, {I}. and {D}urvasula, {R}. and {R}amalho-{O}rtigao, {M}. and {G}tari, {M}. and {Z}hioua, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he {L}eishmania developmental life cycle within its sand fly vector occurs exclusively in the lumen of the insect's digestive tract in the presence of symbiotic bacteria. {T}he composition of the gut microbiota and the factors that influence its composition are currently poorly understood. {A} set of factors, including the host and its environment, may influence this composition. {I}t has been demonstrated that the insect gut microbiota influences the development of several human pathogens, such as {P}lasmodium falciparum. {F}or sand flies and {L}eishmania, understanding the interactions between the parasite and the microbial environment of the vector midgut can provide new tools to control {L}eishmania transmission. {M}ethodology/{P}rincipal findings {T}he midguts of female {P}hlebotomus perniciosus from laboratory colonies or from the field were collected during the months of {J}uly, {S}eptember and {O}ctober 2011 and dissected. {T}he midguts were analyzed by culture-dependent and culture-independent methods. {A} total of 441 and 115 cultivable isolates were assigned to 30 and 11 phylotypes from field-collected and colonized {P}. perniciosus, respectively. {A}nalysis of monthly variations in microbiota composition shows a species diversity decline in {O}ctober, which is to the end of the {L}eishmania infantum transmission period. {I}n parallel, a compilation and a meta-analysis of all available data concerning the microbiota of two {P}sychodidae genera, namely {P}hlebotomus and {L}utzomyia, was performed and compared to {P}. perniciosus, data obtained herein. {T}his integrated analysis did not reveal any substantial divergences between {O}ld and {N}ew world sand flies with regards to the midgut bacterial phyla and genera diversity. {B}ut clearly, most bacterial species (>76%) are sparsely distributed between {P}hlebotominae species. {C}onclusion/{S}ignificance {O}ur results pinpoint the need for a more exhaustive understanding of the bacterial richness and abundance at the species level in {P}hlebotominae sand flies in order to capture the role of midgut bacteria during {L}eishmania development and transmission. {T}he occurrence of {B}acillus subtilis in {P}. perniciosus and at least two other sand fly species studied so far suggests that this bacterial species is a potential candidate for paratransgenic or biolological approaches for the control of sand fly populations in order to prevent {L}eishmania transmission.},
  keywords = {{TUNISIE}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {11},
  numero = {3},
  pages = {e0005484 [22p.]},
  ISSN = {1935-2735},
  year = {2017},
  DOI = {10.1371/journal.pntd.0005484},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070101},
}