@article{fdi:010070092,
  title = {{M}olecular identification of wild triatomines of the genus {R}hodnius in the {B}olivian {A}mazon : strategy and current difficulties},
  author = {{B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique and {C}ondori, {E}. {W}. and {B}uitrago, {R}. and {S}osa, {L}. {F}. and {M}acedo, {C}. {L}. and {B}arnab{\'e}, {C}hristian},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {A}mazon region has recently been considered as endemic in {L}atin {A}merica. {I}n {B}olivia, the vast {A}mazon region is undergoing considerable human migrations and substantial anthropization of the environment, potentially renewing the danger of establishing the transmission of {C}hagas disease. {T}he cases of human oral contamination occurring in 2010 in the town of {G}uayaramerin provided reasons to intensify research. {A}s a result, the goal of this study was to characterize the species of sylvatic triatomines circulating in the surroundings of {Y}ucumo ({B}eni, {B}olivia), a small {A}mazonian city at the foot of the {A}ndes between the capital ({L}a {P}az) and {T}rinidad the largest city of {B}eni. {T}he triatomine captures were performed with mice-baited adhesive traps mostly settled in palm trees in forest fragments and pastures. {S}pecies were identified by morphological observation, dissection of genitalia, and sequencing of three mitochondrial gene fragments and one nuclear fragment. {M}olecular analysis was based on (i) the identity score of the haplotypes with {G}en{B}ank sequences through the {BLAST} algorithm and (ii) construction of phylogenetic trees. {T}hirty-four triatomines, all belonging to the {R}hodnius genus, of which two were adult males, were captured in palm trees in forest fragments and pastures (overall infestation rate, 12.3%). {T}he morphology of the phallic structures in the two males confirmed the {R}. stali species. {F}or the other specimens, after molecular sequencing, only one specimen was identified with confidence as belonging to {R}hodnius robustus, the others belonged to one of the species of the {R}hodnius pictipes complex, probably {R}hodnius stali. {T}he two species, {R}. robustus and {R}. stali, had previously been reported in the {A}lto {B}eni region (edge of the {A}mazon region), but not yet in the {B}eni department situated in the {A}mazon region. {F}urthermore, the difficulties of molecular characterization of closely related species within the three complexes of the genus {R}hodnius are highlighted and discussed.},
  keywords = {{T}riatominae ; {C}hagas ; {M}olecular genetics ; {G}ene ; {S}equencing ; {BOLIVIE} ; {AMAZONIE}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {51},
  numero = {},
  pages = {1--9},
  ISSN = {1567-1348},
  year = {2017},
  DOI = {10.1016/j.meegid.2017.03.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070092},
}