@article{fdi:010070067,
  title = {{E}nantioselective reductive transformation of climbazole : a concept towards quantitative biodegradation assessment in anaerobic biological treatment processes},
  author = {{B}rienza, {M}onica and {C}hiron, {S}erge},
  editor = {},
  language = {{ENG}},
  abstract = {{A}n efficient chiral method-based using liquid chromatography-high resolution-mass spectrometry analytical method has been validated for the determination of climbazole ({CBZ}) enantiomers in waste-water and sludge with quantification limits below the 1 ng/{L} and 2 ng/g range, respectively. {O}n the basis of this newly developed analytical method, the stereochemistry of {CBZ} was investigated over time in sludge biotic and sterile batch experiments under anoxic dark and light conditions and during waste-water biological treatment by subsurface flow constructed wetlands. {CBZ} stereoselective degradation was exclusively observed under biotic conditions, confirming the specificity of enantiomeric fraction variations to biodegradation processes. {A}biotic {CBZ} enantiomerization was insignificant at circumneutral p{H} and {CBZ} was always biotransformed into {CBZ}-alcohol due to the specific and enantioselective reduction of the ketone function of {CBZ} into a secondary alcohol function. {T}his transformation was almost quantitative and biodegradation gave good first order kinetic fit for both enantiomers. {T}he possibility to apply the {R}ayleigh equation to enantioselective {CBZ} biodegradation processes was investigated. {T}he results of enantiomeric enrichment allowed for a quantitative assessment of in situ biodegradation processes due to a good fit ({R}-2 > 0.96) of the anoxic/anaerobic {CBZ} biodegradation to the {R}ayleigh dependency in all the biotic microcosms and was also applied in subsurface flow constructed wetlands. {T}his work extended the concept of applying the {R}ayleigh equation towards quantitative biodegradation assessment of organic contaminants to enantioselective processes operating under anoxic/anaerobic conditions.},
  keywords = {{C}limbazole ; {B}iodegradation ; {E}nantiomeric enrichment ; {R}ayleigh equation},
  booktitle = {},
  journal = {{W}ater {R}esearch},
  volume = {116},
  numero = {},
  pages = {203--210},
  ISSN = {0043-1354},
  year = {2017},
  DOI = {10.1016/j.watres.2017.03.037},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070067},
}