%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sambe-Ba, B.
%A Diallo, M. H.
%A Seck, A.
%A Wane, A. A.
%A Constantin de Magny, Guillaume
%A Boye, C. S. B.
%A Sow, A. I.
%A Gassama-Sow, A.
%T Identification of atypical El Tor V-cholerae O1 Ogawa hosting SXT element in Senegal, Africa
%D 2017
%L fdi:010070052
%G ENG
%J Frontiers in Microbiology
%@ 1664-302X
%K Vibrio cholerae ; O1 virulence ; antibioresistance ; SXT element ; Senegal
%K SENEGAL
%M ISI:000401571100001
%P art. 748 [7 ]
%R 10.3389/fmicb.2017.00748
%U https://www.documentation.ird.fr/hor/fdi:010070052
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-06/010070052.pdf
%V 8
%W Horizon (IRD)
%X Vibrio cholerae O1 is the causative agent of cholera with classical and El Tor, two well-established biotypes. In last 20 years, hybrid strains of classical and El Tor and variant El Tor which carry classical ctxB have emerged worldwide. In 2004-2005, Senegal experienced major cholera epidemic with a number of cases totalling more than 31719 with approximately 458 fatal outcomes (CFR, 1.44%). In this retrospective study, fifty isolates out of a total of 403 V. cholerae biotype El Tor serovar Ogawa isolates from all areas in Senegal during the 2004-2005 cholera outbreak were randomly selected. Isolates were characterized using phenotypic and genotypic methods. The analysis of antibiotic resistance patterns revealed the predominance of the S-Su-TCY-Tsu phenotype (90% of isolates). The molecular characterization of antibiotic resistance revealed the presence of the SXT element, a self-transmissible chromosomally integrating element in all isolates. Most of V. cholerae isolates had an intact virulence cassette (86%) (ctx, zot, ace genes). All isolates tested gave amplification with primers for classical CT, and 10/50 (20%) of isolates carried classical and El Tor ctxB. The study reveals the presence of atypical V. cholerae O1 El Tor during cholera outbreak in Senegal in 2004-2005.
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