Chesnais C. B., Vlaminck J., Kunyu-Shako B., Pion Sébastien, Awaca-Uvon N. P., Weil G. J., Mumba D., Boussinesq Michel. (2016). Measurement of circulating filarial antigen levels in human blood with a point-of-care test strip and a portable spectrodensitometer. American Journal of Tropical Medicine and Hygiene, 94 (6), p. 1324-1329.
Titre du document
Measurement of circulating filarial antigen levels in human blood with a point-of-care test strip and a portable spectrodensitometer
Chesnais C. B., Vlaminck J., Kunyu-Shako B., Pion Sébastien, Awaca-Uvon N. P., Weil G. J., Mumba D., Boussinesq Michel
Source
American Journal of Tropical Medicine and Hygiene, 2016,
94 (6), p. 1324-1329
The Alere Filariasis Test Strip (FTS) is a qualitative, point-of-care diagnostic tool that detects Wuchereria bancrofti circulating filarial antigen (CFA) in human blood, serum, or plasma. The Global Program to Eliminate Lymphatic Filariasis employs the FTS for mapping filariasis-endemic areas and assessing the success of elimination efforts. The objective of this study was to explore the relationship between the intensity of positive test lines obtained by FTS with CFA levels as determined by enzyme-linked immunosorbent assay (ELISA) with blood and plasma samples from 188 individuals who live in a filariasis-endemic area. The intensity of the FTS test line was assessed visually to provide a semiquantitative score (visual Filariasis Test Strip [vFTS]), and line intensity was measured with a portable spectrodensitometer (quantitative Filariasis Test Strip [gFTS]). These results were compared with antigen levels measured by ELISA in plasma from the same subjects. qFTS measurements were highly correlated with vFTS scores (p = 0.94; P < 0.001) and with plasma CFA levels (p = 0.91; P < 0.001). Thus, qFTS assessment is a convenient method for quantifying W bancrofti CFA levels in human blood, which are correlated with adult worm burdens. This tool may be useful for assessing the impact of treatment on adult filarial worms in individuals and communities.
