%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Soussou, S.
%A Brunel, B.
%A Pervent, M.
%A van Tuinen, D.
%A Cleyet-Marel, J. C.
%A Baudoin, Ezékiel
%T Rhizobacterial Pseudomonas spp. strains harbouring acdS gene could enhance metallicolous legume nodulation in Zn/Pb/Cd mine tailings
%D 2017
%L fdi:010069999
%G ENG
%J Water Air and Soil Pollution
%@ 0049-6979
%K Phytostabilisation ; Symbiosis ; Phytostimulation ; Pseudomonas ; PGPR ; ACC ; deaminase
%K FRANCE
%M ISI:000399872600021
%N 4
%P art. 142 [15 ]
%R 10.1007/s11270-017-3309-5
%U https://www.documentation.ird.fr/hor/fdi:010069999
%> https://www.documentation.ird.fr/intranet/publi/2017/05/010069999.pdf
%V 228
%W Horizon (IRD)
%X Phytostabilisation can benefit from phytostimulatory rhizobacteria. Forty-three bacterial strains were isolated from the roots of the metallicolous legume Anthyllis vulneraria ssp. carpatica grown in a highly contaminated mine tailing (total Cd, Pb and Zn were up to 1200; 34,000; and 170,000 mg kg(-1), respectively). We aimed at evaluating their phytostimulatory effects on the development of leguminous metallophytes. Strains were screened for fluorescent siderophores and auxin synthesis, inorganic P solubilisation and 1-amino-cyclopropane-1-carboxylate deaminase (ACCd) activity to define a subset of 11 strains that were inoculated on the leguminous metallophytes A. vulneraria and Lotus corniculatus grown in diluted mine spoil (Zn 34,653; Pb 6842; and Cd 242, all in mg kg-1). All strains were affiliated to Pseudomonas spp. (except two), synthetised auxins and siderophores and solubilised P (except three), and seven of them were ACCd positive. The inoculation effects (shoot-root-nodule biomass, chlorophyll content) depended on legume species and bacterial strain genotype. Phytostimulation scores were unrelated to siderophore/auxin synthesis and P solubilisation rates. Inoculations of the strain nos. 17-43 triggered a 1.2-fold significant increase in the chlorophyll content of A. vulneraria. Chlorophyll content and root biomass of L. corniculatus were significantly increased following the inoculations of the strain nos. 17-22 (1.5-1.4-fold, respectively). The strongest positive effects were related to increases in the nodule biomass of L. corniculatus in the presence of three ACCd-positive strains (1.8-fold), one of which was the highest auxin producer. These data suggest to focus on interactions between ACCd activity and auxin synthesis to enhance nodulation of metallicolous legumes.
%$ 084 ; 076 ; 082 ; 068