Soussou S., Brunel B., Pervent M., van Tuinen D., Cleyet-Marel J. C., Baudoin Ezékiel. (2017). Rhizobacterial Pseudomonas spp. strains harbouring acdS gene could enhance metallicolous legume nodulation in Zn/Pb/Cd mine tailings. Water Air and Soil Pollution, 228 (4), p. art. 142 [15 p.]. ISSN 0049-6979.
Titre du document
Rhizobacterial Pseudomonas spp. strains harbouring acdS gene could enhance metallicolous legume nodulation in Zn/Pb/Cd mine tailings
Soussou S., Brunel B., Pervent M., van Tuinen D., Cleyet-Marel J. C., Baudoin Ezékiel
Source
Water Air and Soil Pollution, 2017,
228 (4), p. art. 142 [15 p.] ISSN 0049-6979
Phytostabilisation can benefit from phytostimulatory rhizobacteria. Forty-three bacterial strains were isolated from the roots of the metallicolous legume Anthyllis vulneraria ssp. carpatica grown in a highly contaminated mine tailing (total Cd, Pb and Zn were up to 1200; 34,000; and 170,000 mg kg(-1), respectively). We aimed at evaluating their phytostimulatory effects on the development of leguminous metallophytes. Strains were screened for fluorescent siderophores and auxin synthesis, inorganic P solubilisation and 1-amino-cyclopropane-1-carboxylate deaminase (ACCd) activity to define a subset of 11 strains that were inoculated on the leguminous metallophytes A. vulneraria and Lotus corniculatus grown in diluted mine spoil (Zn 34,653; Pb 6842; and Cd 242, all in mg kg-1). All strains were affiliated to Pseudomonas spp. (except two), synthetised auxins and siderophores and solubilised P (except three), and seven of them were ACCd positive. The inoculation effects (shoot-root-nodule biomass, chlorophyll content) depended on legume species and bacterial strain genotype. Phytostimulation scores were unrelated to siderophore/auxin synthesis and P solubilisation rates. Inoculations of the strain nos. 17-43 triggered a 1.2-fold significant increase in the chlorophyll content of A. vulneraria. Chlorophyll content and root biomass of L. corniculatus were significantly increased following the inoculations of the strain nos. 17-22 (1.5-1.4-fold, respectively). The strongest positive effects were related to increases in the nodule biomass of L. corniculatus in the presence of three ACCd-positive strains (1.8-fold), one of which was the highest auxin producer. These data suggest to focus on interactions between ACCd activity and auxin synthesis to enhance nodulation of metallicolous legumes.
Plan de classement
Pédologie [068]
;
Sciences du monde végétal [076]
;
Etudes, transformation, conservation du milieu naturel [082]
;
Biotechnologies [084]
