Makiala-Mandanda S., Le Gal F., Ngwaka-Matsung N., Ahuka-Mundeke S., Onanga R., Bivigou-Mboumba B., Pukuta-Simbu E., Gerber A., Abbate J. L., Mwamba D., Berthet N., Leroy Eric, Muyembe-Tamfum J. J., Becquart Pierre. (2017). High prevalence and diversity of hepatitis viruses in suspected cases of yellow fever in the Democratic Republic of Congo. Journal of Clinical Microbiology, 55 (5), p. 1299-1312. ISSN 0095-1137.
Titre du document
High prevalence and diversity of hepatitis viruses in suspected cases of yellow fever in the Democratic Republic of Congo
Année de publication
2017
Auteurs
Makiala-Mandanda S., Le Gal F., Ngwaka-Matsung N., Ahuka-Mundeke S., Onanga R., Bivigou-Mboumba B., Pukuta-Simbu E., Gerber A., Abbate J. L., Mwamba D., Berthet N., Leroy Eric, Muyembe-Tamfum J. J., Becquart Pierre
Source
Journal of Clinical Microbiology, 2017,
55 (5), p. 1299-1312 ISSN 0095-1137
The majority of patients with acute febrile jaundice (>95%) identified through a yellow fever surveillance program in the Democratic Republic of Congo (DRC) test negative for antibodies against yellow fever virus. However, no etiological investigation has ever been carried out on these patients. Here, we tested for hepatitis A (HAV), hepatitis B (HBV), hepatitis C (HCV), hepatitis D (HDV), and hepatitis E (HEV) viruses, all of which can cause acute febrile jaundice, in patients included in the yellow fever surveillance program in the DRC. On a total of 498 serum samples collected from suspected cases of yellow fever from January 2003 to January 2012, enzyme-linked immunosorbent assay (ELISA) techniques were used to screen for antibodies against HAV (IgM) and HEV (IgM) and for antigens and antibodies against HBV (HBsAg and anti-hepatitis B core protein [HBc] IgM, respectively), HCV, and HDV. Viral loads and genotypes were determined for HBV and HVD. Viral hepatitis serological markers were diagnosed in 218 (43.7%) patients. The seroprevalences were 16.7% for HAV, 24.6% for HBV, 2.3% for HCV, and 10.4% for HEV, and 26.1% of HBV-positive patients were also infected with HDV. Median viral loads were 4.19 x 10(5) IU/ml for HBV (range, 769 to 9.82 x 10(9) IU/ml) and 1.4 x 10(6) IU/ml for HDV (range, 3.1 x 10(2) to 2.9 x 10(8) IU/ml). Genotypes A, E, and D of HBV and genotype 1 of HDV were detected. These high hepatitis prevalence rates highlight the necessity to include screening for hepatitis viruses in the yellow fever surveillance program in the DRC.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
REPUBLIQUE DEMOCRATIQUE DU CONGO
Localisation
Fonds IRD [F B010069985]
Identifiant IRD
fdi:010069985
