@article{fdi:010069609,
  title = {{R}educed turn-around time for {M}ycobacterium tuberculosis drug susceptibility testing with a proportional agar microplate assay},
  author = {{N}guyen, {V}. {A}. {T}. and {N}guyen, {H}. {Q}. and {V}u, {T}. {T}. and {N}guyen, {N}. {A}. {T}. and {D}uong, {C}. {M}. and {T}ran, {T}. {H}. {T}. and {N}guyen, {H}. {V}. and {D}ang, {D}. {A}. and {B}anuls, {A}nne-{L}aure},
  editor = {},
  language = {{ENG}},
  abstract = {{M}ultidrug-resistant tuberculosis is a major issue worldwide; however, accessibility to drug susceptibility testing ({DST}) is still limited in developing countries, owing to high costs and complexity. {W}e developed a proportion method on 12-well microplates for {DST}. {T}he assay reduced the time to results to <12 days and <10 days when bacterial growth was checked with the naked eye or a microscope, respectively. {C}omparison with the {C}anetti-{G}rosset method showed that the results of the two assays almost overlapped (kappa index 0.98 (95% {CI} 0.91-1.00) for isoniazid, rifampicin, streptomycin; and kappa index 0.92 (95% {CI} 0.85-0.99) for ethambutol). {T}he sequencing of genes involved in drug resistance showed similar level of phenotype-genotype agreement between techniques. {F}inally, measurement of the {MIC}s of rifampicin and ethambutol suggests that the currently used critical ethambutol concentration should be revised, and that the current molecular drug susceptibility tests for rifampicin need to be re-evaluated, as in vitro rifampicin-sensitive isolates could harbour drug resistance-associated mutation(s). {C}linical {M}icrobiology and {I}nfection ({C}) 2015 {E}uropean {S}ociety of {C}linical {M}icrobiology and {I}nfectious {D}iseases. {P}ublished by {E}lsevier {L}td. {A}ll rights reserved.},
  keywords = {{D}rug resistance ; drug susceptibility testing ; {M}. tuberculosis ; {MIC} ; proportional microplate assay ; sequencing},
  booktitle = {},
  journal = {{C}linical {M}icrobiology and {I}nfection},
  volume = {21},
  numero = {12},
  pages = {1084--1092},
  ISSN = {1198-743{X}},
  year = {2015},
  DOI = {10.1016/j.cmi.2015.08.024},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069609},
}