@article{fdi:010069512,
  title = {{D}eep sequencing is an appropriate tool for the selection of unique {H}epatitis {C} virus ({HCV}) variants after single genomic amplification},
  author = {{G}uinoiseau, {T}. and {M}oreau, {A}. and {H}ohnadel, {G}. and {N}go-{G}iang-{H}uong, {N}icole and {B}rulard, {C}. and {V}ourc'h, {P}. and {G}oudeau, {A}. and {G}audy-{G}raffin, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}epatitis {C} virus ({HCV}) evolves rapidly in a single host and circulates as a quasispecies wich is a complex mixture of genetically distinct virus's but closely related namely variants. {T}o identify intra-individual diversity and investigate their functional properties in vitro, it is necessary to define their quasispecies composition and isolate the {HCV} variants. {T}his is possible using single genome amplification ({SGA}). {T}his technique, based on serially diluted c{DNA} to amplify a single c{DNA} molecule (clonal amplicon), has already been used to determine individual {HCV} diversity. {I}n these studies, positive {PCR} reactions from {SGA} were directly sequenced using {S}anger technology. {T}he detection of non-clonal amplicons is necessary for excluding them to facilitate further functional analysis. {H}ere, we compared {N}ext {G}eneration {S}equencing ({NGS}) with {D}e {N}ovo assembly and {S}anger sequencing for their ability to distinguish clonal and non-clonal amplicons after {SGA} on one plasma specimen. {A}ll amplicons (n = 42) classified as clonal by {NGS} were also classified as clonal by {S}anger sequencing. {N}o double peaks were seen on electropherograms for non-clonal amplicons with position-specific nucleotide variation below 15% by {NGS}. {A}ltogether, {NGS} circumvented many of the difficulties encountered when using {S}anger sequencing after {SGA} and is an appropriate tool to reliability select clonal amplicons for further functional studies.},
  keywords = {{THAILANDE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {12},
  numero = {3},
  pages = {e0174852 [11 p.]},
  ISSN = {1932-6203},
  year = {2017},
  DOI = {10.1371/journal.pone.0174852},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069512},
}