@article{fdi:010069472,
  title = {{C}omparative genomics of {G}lossina palpalis gambiensis and {G}. morsitans morsitans to reveal gene orthologs involved in infection by {T}rypanosoma brucei gambiense},
  author = {{S}oumana, {I}. {H}. and {T}chicaya, {B}. and {R}ialle, {S}. and {P}arrinello, {H}. and {G}eiger, {A}nne},
  editor = {},
  language = {{ENG}},
  abstract = {{B}lood-feeding {G}lossina palpalis gambiense ({G}pg) fly transmits the single- celled eukaryotic parasite {T}rypanosoma brucei gambiense ({T}bg), the second {G}lossina fly {A}frican trypanosome pair being {G}lossina morsitans/{T}.brucei rhodesiense. {W}hatever the {T}. brucei subspecies, whereas the onset of their developmental program in the zoo-anthropophilic blood feeding flies does unfold in the fly midgut, its completion is taking place in the fly salivary gland where does emerge a low size metacyclic trypomastigote population displaying features that account for its establishment in mammals-human individuals included. {C}onsidering that the two {G}lossina-{T}. brucei pairs introduced above share similarity with respect to the developmental program of this {A}frican parasite, we were curious to map on the {G}lossina morsitans morsitans ({G}mm), the {D}ifferentially {E}xpressed {G}enes ({DEG}s) we listed in a previous study. {B}riefly, using the gut samples collected at days 3, 10, and 20 from {G}pg that were fed or not at day 0 on {T}bg-hosting mice, these {DGE} lists were obtained from {RNA} seq-based approaches. {H}ere, post the mapping on the quality controlled {DEG}s on the {G}mm genome, the identified ortholog genes were further annotated, the resulting datasets being compared. {A}round 50% of the {G}pg {DEG}s were shown to have orthologs in the {G}mm genome. {U}nder one of the three {G}lossina midgut sampling conditions, the number of {DEG}s was even higher when mapping on the {G}mm genome than initially recorded. {M}any {G}mm genes annotated as "{H}ypothetical" were mapped and annotated on many distinct databases allowing some of them to be properly identified. {W}e identify {G}lossina fly candidate genes encoding (a) a broad panel of proteases as well as (b) chitin-binding proteins, (c) antimicrobial peptide production-{P}ro3 protein, transferrin, mucin, atttacin, cecropin, etc-to further select in functional studies, the objectives being to probe and validated fly genome manipulation that prevents the onset of the developmental program of one or the other {T}. brucei spp. stumpy form sampled by one of the other bloodfeeding {G}lossina subspecies.},
  keywords = {human {A}frican {T}rypanosomiasis ; {G}lossina palpalis gambiensis ; {G}lossina morsitans morsitans ; {T}rypanosoma brucei gambiense ; differentially ; expressed genes ; heterologous genes},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {8},
  numero = {},
  pages = {art. 540 [20 p.]},
  ISSN = {1664-302{X}},
  year = {2017},
  DOI = {10.3389/fmicb.2017.00540},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069472},
}