%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Di Lorenzo, F.
%A Palmigiano, A.
%A Al Bitar-Nehme, S.
%A Sturiale, L.
%A Duda, K. A.
%A Gully, Djamel
%A Lanzetta, R.
%A Giraud, Eric
%A Garozzo, D.
%A Bernardini, M. L.
%A Molinaro, A.
%A Silipo, A.
%T The LipidA from Rhodopseudomonas palustris strain BisA53 LPS possesses a unique structure and low immunostimulant properties
%D 2017
%L fdi:010069463
%G ENG
%J Chemistry : European Journal
%@ 0947-6539
%K fatty acids ; glycolipids ; innate immunity ; lipopolysaccharides ; NMR ; spectroscopy
%M ISI:000397502900016
%N 15
%P 3637-3647
%R 10.1002/chem.201604379
%U https://www.documentation.ird.fr/hor/fdi:010069463
%> https://www.documentation.ird.fr/intranet/publi/2017/04/010069463.pdf
%V 23
%W Horizon (IRD)
%X The search for novel lipidA analogues from any biological source that can act as antagonists, displaying inhibitory activity towards the production of pro-inflammatory cytokines, or as immunomodulators in mammals, is a very topical issue. To this aim, the structure and immunological properties of the lipopolysaccharide lipidA from the purple nonsulfur bacterium Rhodopseudomonas palustris strain BisA53 have been determined. This lipidA displays a unique structural feature, with a non-phosphorylated skeleton made up of the tetrasaccharide Manp--(14)-GlcpN3N--16-GlcpN3N--(11)--GalpA, and four primary amide-linked 14:0(3-OH) and, as secondary O-acyl substituents, a 16:0 and the very long-chain fatty acid 26:0(25-OAc), appended on the GlcpN3N units. This lipidA architecture is definitely rare, so far identified only in the genus Bradyrhizobium. Immunological tests on both murine bone-marrow-derived and human monocyte-derived macrophages revealed an extremely low immunostimulant capability of this LPS lipidA.
%$ 020 ; 084