@article{fdi:010069460,
  title = {{S}erological and molecular identification of {L}eptospira spp. in swine and stray dogs from {M}alaysia},
  author = {{B}enacer, {D}. and {T}hong, {K}. {L}. and {O}oi, {P}. {T}. and {S}ouris, {M}arc and {L}ewis, {J}. {W}. and {A}hmed, {A}. {A}. and {Z}ain, {S}. {N}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{L}eptospirosis is endemic in {M}alaysia with {L}eptospira species extensively isolated from domestic and wild animals. {R}ats were found to be the principal maintenance hosts followed by cattle, pigs, and dogs. {T}he objectives of this study were to isolate and identify {L}eptospira serovars circulating among swine from three different farms and also from stray dogs and cats from {K}lang valley, {S}elangor, {M}alaysia. {U}rine and kidney samples collected from 150 stray dogs, 50 cats and 81 swine were inoculated into semi-solid {E}llinghausen {M}c{C}ullough {J}ohnson and {H}arris ({EMJH}) media supplemented with additional 5-{F}luorouracil. {D}ark field microscopy revealed only one positive culture of {L}eptospira from dog and swine samples, but all cat samples were negative. {T}he {PCR} technique using published primers detected 11 positives in urine samples of dogs and 5 positives from swine. {T}he microscopic agglutination test ({MAT}) confirmed the presence of two serovars in both dog and swine populations namely, {L}. interrogans serovar {C}anicola and {L}. interrogans serovar {P}omona ({MAT} >= 100), with {N}ot {I}-{PFGE} analyses separating these two serovars into distinct profiles. {D}espite the low prevalence in stray dogs, the latter may play an important role in the contamination of the environment. {S}wine can also pose a potential risk of infection to humans and other domestic animals, especially those living close to swine farms. {T}hus improving hygiene and eradication of rodents in swine farms are likely to reduce the risk of infection.},
  keywords = {{MALAISIE}},
  booktitle = {},
  journal = {{T}ropical {B}iomedicine},
  volume = {34},
  numero = {1},
  pages = {89--97},
  ISSN = {0127-5720},
  year = {2017},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069460},
}