@article{fdi:010069449,
  title = {{P}referential expression of domain cassettes 4, 8 and 13 of {P}lasmodium falciparum erythrocyte membrane protein 1 in severe malaria imported in {F}rance},
  author = {{A}rgy, {N}. and {B}ertin, {G}wladys and {M}ilet, {J}acqueline and {H}ubert, {V}. and {C}lain, {J}. and {C}ojean, {S}. and {H}ouze, {P}. and {T}uikue {N}dam, {N}icaise and {K}endjo, {E}. and {D}eloron, {P}hilippe and {H}ouze, {S}. and {CNRP} {S}tudy {G}roup},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjectives: {S}evere {P}lasmodium falciparum malaria ({SM}) involves cytoadhesion of parasitized red blood cells, mediated by {P}. falciparum erythrocyte membrane protein 1, which is encoded by var genes. {E}xpression of var gene group {A} and {B} or encoding domain cassettes {DC}4, {DC}5, {DC}8 and {DC}13 has been implicated in {SM} in {A}frican children, but no data exist in the context of imported malaria. {T}he aim of this study was to investigate var gene expression linked to clinical presentation and host factors in {SM} imported into {F}rance. {M}ethods: {E}xpression level of var gene groups {A}, {B}, {C}, var1, var2csa, var3 and var genes encoding {DC}4, {DC}5, {DC}8 and {DC}13 was measured by quantitative {RT}- {PCR} and expressed in transcript units. {S}eventy {SM} and 48 uncomplicated malaria ({UM}) {P}. falciparum cases were analysed according to disease severity, epidemiological characteristics (migrants or travellers) and anti- {P}. falciparum antibodies. {C}luster analysis was performed to identify gene expression profiles. {R}esults: {V}ar1 and {B}/ {C} expression were higher in {UM} than {SM} (0.66 (0-1.1) and 1.88 (1.3-2.4); p < 0.04, respectively). {G}roup {C} expression differed between migrants and travellers (0.21 (0-0.75) versus 0 (0e0); p 0.002). {G}roup {A} differed in naive and pre- exposed patients (1.1 (0.7-1.5) versus 0.4 (0-1.1); p 0.01). {P}opulation clusters revealed increased expression from group {A} and {B} var genes, and {DC}4, {DC}8 and {DC}13 in {SM}. {C}onclusions: {T}hese results corroborate the implication of {DC}4, {DC}8 and {DC}13 in severe imported malaria cases as {A}frican children, and their expression depends of host factors.},
  keywords = {{D}omain cassette ; {DC}4 ; {DC}8 ; {DC}13 ; {I}mported malaria ; {S}evere malaria ; {V}ar ; gene ; {FRANCE}},
  booktitle = {},
  journal = {{C}linical {M}icrobiology and {I}nfection},
  volume = {23},
  numero = {3},
  pages = {211.e1–211.e4},
  ISSN = {1198-743{X}},
  year = {2017},
  DOI = {10.1016/j.cmi.2016.10.012},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069449},
}