Ben Hania W., Joseph Manon, Bunk B., Sproer C., Klenk H. P., Fardeau Marie-Laure, Spring S. (2017). Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria. Environmental Microbiology, 19 (3), p. 1134-1148. ISSN 1462-2912.
Titre du document
Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria
Ben Hania W., Joseph Manon, Bunk B., Sproer C., Klenk H. P., Fardeau Marie-Laure, Spring S.
Source
Environmental Microbiology, 2017,
19 (3), p. 1134-1148 ISSN 1462-2912
The anaerobic, mesophilic and moderately halophilic strain L21-Spi-D4(T) was recently isolated from the suboxic zone of a hypersaline cyanobacterial mat using protein-rich extracts of Arthrospira (formerly Spirulina) platensis as substrate. Phylogenetic analyses based on 16S rRNA genes indicated an affiliation of the novel strain with the Bacteroidetes clade MgMjR-022, which is widely distributed and abundant in hypersaline microbial mats and heretofore comprised only sequences of uncultured bacteria. Analyses of the complete genome sequence of strain L21-Spi-D4(T) revealed a possible specialization on the degradation of cyanobacterial biomass. Besides genes for enzymes degrading specific cyanobacterial proteins a conspicuous transport complex for the polypeptide cyanophycin could be identified that is homologous to typical polysaccharide utilization loci of Bacteroidetes. A distinct and reproducible co-occurrence pattern of environmental 16S rRNA gene sequences of the MgMjR-022 clade and cyanobacteria in the suboxic zone of hypersaline mats points to a specific dependence of members of this clade on decaying cyanobacteria. Based on a comparative analysis of phenotypic, genomic and ecological characteristics we propose to establish the novel taxa Salinivirga cyanobacteriivorans gen. nov., sp. nov., represented by the type strain L21-Spi-D4(T), and Salinivirgaceae fam. nov., comprising sequences of the MgMjR-022 clade.
