@article{fdi:010069413,
  title = {{R}eemergence of chloroquine-sensitive pfcrt {K}76 {P}lasmodium falciparum genotype in southeastern {C}ameroon},
  author = {{T}uikue {N}dam, {N}icaise and {B}asco, {L}eonardo and {N}gane, {V}. {F}. and {A}youba, {A}hidjo and {N}golle, {E}. {M}. and {D}eloron, {P}hilippe and {P}eeters, {M}artine and {T}ahar, {R}achida},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}hloroquine had been used extensively during the last five decades in {C}ameroon. {I}ts decreasing clinical effectiveness, supported by high proportions of clinical isolates carrying the mutant pfcrt haplotype ({CVIET}), led the health authorities to resort to amodiaquine monotherapy in 2002 and artemisinin-based combination therapy ({ACT}) in 2004 (artesunate-amodiaquine, with artemether-lumefantrine as an alternative since 2006) as the first-line treatment of uncomplicated malaria. {T}he aim of the present study was to investigate whether the withdrawal of chloroquine was associated with a reduction in pfcrt mutant parasite population and reemergence of chloroquinesensitive parasites in southeastern {C}ameroon between 2003 and 2012. {M}ethods: {T}he frequency of pfcrt haplotypes at positions 72-76 in {P}lasmodium falciparum isolates collected from individuals in 2003 and 2012 in southeastern {C}ameroon was determined by sequence specific oligonucleotide probes-enzyme linked immunosorbent assay ({SSOP}-{ELISA}). {R}esults: {T}he proportions of parasites carrying the mutant haplotype {CVIET} and the wild-type {CVMNK} were 53.0 and 28.0% in 2003, respectively. {T}he proportion of the mutant haplotype in samples collected 9 years later decreased to 25.3% whereas the proportion of parasites carrying the wild-type {CVMNK} haplotype was 53.7%. {C}onclusions: {E}ven though the proportion of chloroquine-sensitive parasites seems to be increasing in southeastern {C}ameroon, a reintroduction of chloroquine cannot be recommended at present in {C}ameroon. {T}he current national anti-malarial drug policy should be implemented and reinforced to combat drug-resistant malaria.},
  keywords = {{D}rug resistance ; {C}hloroquine ; {A}rtemisinin ; {M}olecular markers ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {16},
  numero = {},
  pages = {art. 130 [6 p.]},
  ISSN = {1475-2875},
  year = {2017},
  DOI = {10.1186/s12936-017-1783-2},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069413},
}