@article{fdi:010069390,
  title = {{S}equencing the extrachromosomal circular mobilome reveals retrotransposon activity in plants},
  author = {{L}anciano, {S}ophie and {C}arpentier, {M}. {C}. and {L}lauro, {C}. and {J}obet, {E}. and {R}obakowska-{H}yzorek, {D}. and {L}asserre, {E}. and {G}hesqui{\`e}re, {A}lain and {P}anaud, {O}. and {M}irouze, {M}arie},
  editor = {},
  language = {{ENG}},
  abstract = {{R}etrotransposons are mobile genetic elements abundant in plant and animal genomes. {W}hile efficiently silenced by the epigenetic machinery, they can be reactivated upon stress or during development. {T}heir level of transcription not reflecting their transposition ability, it is thus difficult to evaluate their contribution to the active mobilome. {H}ere we applied a simple methodology based on the high throughput sequencing of extrachromosomal circular {DNA} (ecc{DNA}) forms of active retrotransposons to characterize the repertoire of mobile retrotransposons in plants. {T}his method successfully identified known active retrotransposons in both {A}rabidopsis and rice material where the epigenome is destabilized. {W}hen applying mobilome-seq to developmental stages in wild type rice, we identified {P}op{R}ice as a highly active retrotransposon producing ecc{DNA} forms in the wild type endosperm. {T}he mobilome-seq strategy opens new routes for the characterization of a yet unexplored fraction of plant genomes.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {G}enetics},
  volume = {13},
  numero = {2},
  pages = {e1006630 [20p.]},
  ISSN = {1553-7404},
  year = {2017},
  DOI = {10.1371/journal.pgen.1006630},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069390},
}