@article{fdi:010069377,
  title = {{N}o excess of cis-regulatory variation associated with intraspecific selection in wild pearl millet ({C}enchrus americanus)},
  author = {{R}hon{\'e}, {B}{\'e}n{\'e}dicte and {M}ariac, {C}{\'e}dric and {C}ouderc, {M}arie and {B}erthouly, {C}{\'e}cile and {O}usseini, {I}. {S}. and {V}igouroux, {Y}ves},
  editor = {},
  language = {{ENG}},
  abstract = {{S}everal studies suggestthat cis-regulatory mutations are the favorite target of evolutionary changes, one reason being that cis-regulatory mutations might have fewer deleterious pleiotropic effects than protein-coding mutations. {A} review of the process also suggests that this bias towards adaptive cis-regulatory variation might be less pronounced at the intraspecific level compared with the interspecific level. {I}n this study, we assessed the contribution of cis-regulatory variation to adaptation at the intraspecific level using populations of wild pearl millet ({C}enchrus americanus ssp. monodii) sampled along an environmental gradient in {N}iger. {F}rom {RNA} sequencing of hybrids to assess allele-specific expression, we identified genes with cis-regulatory divergence between two parental accessions collected in contrasted environmental conditions. {T}his revealed that similar to 15% of transcribed genes showed cis-regulatory variation. {I}ntersecting the gene set exhibiting cis-regulatory variation with the gene set identified as targets of selection revealed no excess of cis-acting mutations among the selected genes. {W}e additionally found no excess of cis-regulatory variation among genes associated with adaptive traits. {A}s our approach relied on methods identifying mainly genes submitted to strong selection pressure or with high phenotypic effect, the contribution of cis-regulatory changes to soft selection or polygenic adaptive traits remains to be tested. {H}owever our results favor the hypothesis that enrichment of adaptive cis-regulatory divergence builds up over time. {F}or short evolutionary time-scales, cis-acting mutations are not predominantly involved in adaptive evolution associated with strong selective signal.},
  keywords = {allele-specific expression ; cis-regulation ; environmental gradient ; local adaptation ; {C}enchrus americanus ; selection ; {NIGER}},
  booktitle = {},
  journal = {{G}enome {B}iology and {E}volution},
  volume = {9},
  numero = {2},
  pages = {388--397},
  ISSN = {1759-6653},
  year = {2017},
  DOI = {10.1093/gbe/evx004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069377},
}