@article{fdi:010069262,
  title = {{L}ive-cell analysis of {DNA} methylation during sexual reproduction in {A}rabidopsis reveals context and sex-specific dynamics controlled by noncanonical {R}d{DM}},
  author = {{I}ngouff, {M}. and {S}elles, {B}enjamin and {M}ichaud, {C}aroline and {V}u, {T}. {M}. and {B}erger, {F}. and {S}chorn, {A}. {J}. and {A}utran, {D}aphn{\'e} and {V}an {D}urme, {M}. and {N}owack, {M}. {K}. and {M}artienssen, {R}. {A}. and {G}rimanelli, {D}aniel},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. {W}hile relatively stable during somatic growth, {DNA} methylation is reprogrammed genome-wide during mammalian reproduction. {R}eprogramming is essential for zygotic totipotency and to prevent transgenerational inheritance of epimutations. {H}owever, the extent of {DNA} methylation reprogramming in plants remains unclear. {H}ere, we developed sensors reporting with single-cell resolution {CG} and non-{CG} methylation in {A}rabidopsis. {L}ive imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. {W}e found that {CHH} methylation in the egg cell depends on {DOMAINS} {REARRANGED} {METHYLASE} 2 ({DRM}2) and {RNA} polymerase {V} ({P}ol {V}), two main actors of {RNA}-directed {DNA} methylation, but does not depend on {P}ol {IV}. {O}ur sensors provide insight into global {DNA} methylation dynamics at the single-cell level with high temporal resolution and offer a powerful tool to track {CG} and non-{CG} methylation both during development and in response to environmental cues in all organisms with methylated {DNA}, as we illustrate in mouse embryonic stem cells.},
  keywords = {{DNA} methylation ; sensors ; reprogramming ; reproduction},
  booktitle = {},
  journal = {{G}enes and {D}evelopment},
  volume = {31},
  numero = {1},
  pages = {72--83},
  ISSN = {0890-9369},
  year = {2017},
  DOI = {10.1101/gad.289397.116},
  URL = {https://www.documentation.ird.fr/hor/fdi:010069262},
}