%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Frikha-Dammak, D.
%A Fardeau, Marie-Laure
%A Cayol, J. L.
%A Ben Fguira-Fourati, L.
%A Najeh, S.
%A Ollivier, Bernard
%A Maalej, S.
%T Paludifilum halophilum gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern
%D 2016
%L fdi:010069256
%G ENG
%J International Journal of Systematic and Evolutionary Microbiology
%@ 1466-5026
%K TUNISIE ; SFAX
%M ISI:000393357900073
%N 12
%P 5371-5378
%R 10.1099/ijsem.0.001523
%U https://www.documentation.ird.fr/hor/fdi:010069256
%> https://www.documentation.ird.fr/intranet/publi/2017/03/010069256.pdf
%V 66
%W Horizon (IRD)
%X A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3(T) was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase- and oxidase-positive. Optimum growth occurred at 40-45 degrees C, with 10 % (w/v) NaCl and at pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3(T) belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa DSM 45169(T) 94.2 % with Kroppenstedtia eburnea DSM 45196(T), 94.3 % with Kroppenstedtia guangzhouensis KCTC 29149(T), 94.3 % with Melghirimyces algeriensisDSM 45474(T) and 94.5 % with Salinithrix halophila CECT 8506(T). The predominant menaquinone is MK-7, but MK-8 and some minor unidentified components are also present in trace amounts. The major cellular fatty acids are anteiso-C-15:0, iso-C-15:0 and iso-C-17:0. In addition to four major polar lipids identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmethylethanolamine, five minor unknown lipids were detected in cell membranes. The DNA G+C content of strain SMBg3(T) is 51.2 mol%. Strain SMBg3(T) is distinct from recognized genera of the family Thermoactinomycetaceae by morphological, biochemical and chemotaxonomic characteristics. On the basis of physiological and phylogenetic data, strain SMBg3(T) represents a novel species of a new genus in the family Thermoactinomycetaceae for which the name Paludifilum halophilum gen. nov., sp. nov. is proposed. The type strain of the type species is SMBg3(T) (=DSM 102817(T)=CCUG 68698(T)).
%$ 084