Publications des scientifiques de l'IRD

Frikha-Dammak D., Fardeau Marie-Laure, Cayol J. L., Ben Fguira-Fourati L., Najeh S., Ollivier Bernard, Maalej S. (2016). Paludifilum halophilum gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern. International Journal of Systematic and Evolutionary Microbiology, 66 (12), p. 5371-5378. ISSN 1466-5026.

Titre du document
Paludifilum halophilum gen. nov., sp. nov., a thermoactinomycete isolated from superficial sediment of a solar saltern
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000393357900073
Auteurs
Frikha-Dammak D., Fardeau Marie-Laure, Cayol J. L., Ben Fguira-Fourati L., Najeh S., Ollivier Bernard, Maalej S.
Source
International Journal of Systematic and Evolutionary Microbiology, 2016, 66 (12), p. 5371-5378 ISSN 1466-5026
A novel filamentous, halophilic, thermotolerant bacterium, strain SMBg3(T) was isolated from superficial sediment of a solar saltern in Sfax, Tunisia. The isolate is Gram-staining-positive, aerobic, catalase- and oxidase-positive. Optimum growth occurred at 40-45 degrees C, with 10 % (w/v) NaCl and at pH 8.0-9.0. Long and well developed aerial and substrate mycelia, with long chains of fluorescent and circular spores, were observed on all tested media. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMBg3(T) belongs to an independent phylogenetic lineage of the family Thermoactinomycetaceae and shows a gene sequence similarity of 94 % with Desmospora activa DSM 45169(T) 94.2 % with Kroppenstedtia eburnea DSM 45196(T), 94.3 % with Kroppenstedtia guangzhouensis KCTC 29149(T), 94.3 % with Melghirimyces algeriensisDSM 45474(T) and 94.5 % with Salinithrix halophila CECT 8506(T). The predominant menaquinone is MK-7, but MK-8 and some minor unidentified components are also present in trace amounts. The major cellular fatty acids are anteiso-C-15:0, iso-C-15:0 and iso-C-17:0. In addition to four major polar lipids identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmethylethanolamine, five minor unknown lipids were detected in cell membranes. The DNA G+C content of strain SMBg3(T) is 51.2 mol%. Strain SMBg3(T) is distinct from recognized genera of the family Thermoactinomycetaceae by morphological, biochemical and chemotaxonomic characteristics. On the basis of physiological and phylogenetic data, strain SMBg3(T) represents a novel species of a new genus in the family Thermoactinomycetaceae for which the name Paludifilum halophilum gen. nov., sp. nov. is proposed. The type strain of the type species is SMBg3(T) (=DSM 102817(T)=CCUG 68698(T)).
Plan de classement
Biotechnologies [084]
Description Géographique
TUNISIE ; SFAX
Localisation
Fonds IRD [F B010069256]
Identifiant IRD
fdi:010069256
Contact