@article{fdi:010068934,
  title = {{P}rocarcinogens - {D}etermination and evaluation by yeast-based biosensor transformed with plasmids incorporating {RAD}54 reporter construct and cytochrome {P}450 genes},
  author = {{V}an {N}goc {B}ui, and {T}hi {T}hu {H}uyen {N}guyen, and {C}hi {T}hanh {M}ai, and {B}ettarel, {Y}van and {T}hi {Y}en {H}oang, and {T}hi {T}huy {L}inh {T}rinh, and {N}am {H}ai {T}ruong, and {H}oang {H}a {C}hu, and {V}u {T}hanh {T}hanh {N}guyen, and {H}uu {D}uc {N}guyen, and {W}olfl, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n {V}ietnam, a great number of toxic substances, including carcinogens and procarcinogens, from industrial and agricultural activities, food production, and healthcare services are daily released into the environment. {I}n the present study, we report the development of novel yeast-based biosensor systems to determine both genotoxic carcinogens and procarcinogens by cotransformation with two plasmids. {O}ne plasmid is carrying human {CPR} and {CYP} ({CYP}3{A}4, {CYP}2{B}6, or {CYP}2{D}6) genes, while the other contains the {RAD}54-{GFP} reporter construct. {T}he three resulting coexpression systems bearing both {CPR}-{CYP} and {RAD}54-{GFP} expression cassettes were designated as {CYP}3{A}4/{CYP}2{B}6/{CYP}2{D}6 + {RAD}54 systems, respectively and used to detect and evaluate the genotoxic potential of carcinogens and procarcinogens by selective activation and induction of both {CPR}-{CYP} and {RAD}54-{GFP} expression cassettes in response to {DNA} damage. {P}rocarcinogens were shown to be predominantly, moderately or not bioactivated by one of the {CYP} enzymes and thus selectively detected by the specific coexpression system. {A}flatoxin {B}-1 and benzo(a) pyrene were predominantly detected by the {CYP}3{A}4 + {RAD}54 system, while {N}-nitrosodimethylamine only moderately activated the {CYP}2{B}6 + {RAD}54 reporter system and none of them was identified by the {CYP}2{D}6 + {RAD}54 system. {I}n contrast, the genotoxic carcinogen, methyl methanesulfonate, was detected by all systems. {O}ur yeast-reporter system can be performed in 384-well microplates to provide efficient genotoxicity testing to identify various carcinogenic compounds and reduce chemical consumption to about 53% as compared with existing 96-well genotoxicity bioassays. {I}n association with a liquid handling robot, this platform enables rapid, cost-effective, and high-throughput screening of numerous analytes in a fully automated and continuous manner without the need for user interaction.},
  keywords = {{VIET} {NAM}},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {11},
  numero = {12},
  pages = {e0168721 [18 ]},
  ISSN = {1932-6203},
  year = {2016},
  DOI = {10.1371/journal.pone.0168721},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068934},
}