Publications des scientifiques de l'IRD

Zhang J. J., Yang X., Guo C., Lajudie Philippe de, Singh R. P., Wang E. T., Chen W. F. (2017). Mesorhizobium muleiense and Mesorhizobium gsp nov are symbionts of Cicer arietinum L. in alkaline soils of Gansu, Northwest China. Plant and Soil, 410 (1-2), p. 103-112. ISSN 0032-079X.

Titre du document
Mesorhizobium muleiense and Mesorhizobium gsp nov are symbionts of Cicer arietinum L. in alkaline soils of Gansu, Northwest China
Année de publication
2017
Type de document
Article référencé dans le Web of Science WOS:000392392200008
Auteurs
Zhang J. J., Yang X., Guo C., Lajudie Philippe de, Singh R. P., Wang E. T., Chen W. F.
Source
Plant and Soil, 2017, 410 (1-2), p. 103-112 ISSN 0032-079X
To describe the chickpea rhizobial diversity in Gansu Province of China, so far uninvestigated. Nodule bacteria were isolated from chickpea grown in Gansu and characterized by phylogenetic analysis of 16S rRNA gene, housekeeping genes (atpD, recA, and glnII), and symbiotic genes (nodC and nifH). The genetic differentiation and gene flow among the chickpea microsymbionts were estimated and considered in view of soil characteristics. A total of nineteen new isolates originating from alkaline-saline (pH 7.9-8.1) soils in Gansu formed two clades by housekeeping gene analyses; clade I (16 isolates) was identified as Mesorhizobium muleiense (97.7-98.7 % of similarities with the type strain CCBAU 83963(T)) and clade II (3 isolates) formed a separate group within Mesorhizobium branch, displaying 97.0 and 96.6 % similarities with the most related type strains M. temperatum SDW 018(T) and M. muleiense CCBAU 83963(T), respectively. Their symbiotic genes (nodC and nifH) were highly similar (> 98.0 %) with those described for chickpea microsymbionts in literature, i.e., M. muleiense, M. ciceri, and M. mediterraneum. The genetic differentiation and gene flow between the chickpea rhizobial groups supported our conclusions for species affiliation. M. muleiense and a novel Mesorhizobium genospecies, sharing similar symbiotic genes, are distributed in the alkaline-saline soils of Gansu and may be considered as the main and specific chickpea symbionts in the Northwest of China.
Plan de classement
Biotechnologies [084]
Description Géographique
CHINE
Localisation
Fonds IRD [F B010068933]
Identifiant IRD
fdi:010068933
Contact