@article{fdi:010068916,
  title = {{T}ransmission of {L}eishmania infantum in the canine leishmaniasis focus of {M}ont-{R}olland, {S}enegal : ecological, parasitological and molecular evidence for a possible role of {S}ergentomyia sand flies},
  author = {{S}enghor, {M}. {W}. and {N}iang, {A}. {A}. and {D}epaquit, {J}. and {F}erte, {H}. and {F}aye, {M}. {N}. and {E}lguero, {E}ric and {G}aye, {O}. and {A}lten, {B}. and {P}erktas, {U}. and {C}assan, {C}{\'e}cile and {F}aye, {B}. and {B}anuls, {A}nne-{L}aure},
  editor = {},
  language = {{ENG}},
  abstract = {{L}eishmania ({L}.) infantum is the causative agent in an endemic focus of canine leishmaniasis in the {M}ont-{R}olland district ({T}hies, {S}enegal). {I}n this area, the transmission cycle is well established and more than 30% of dogs and 20% of humans are seropositive for {L}. infantum. {H}owever, the sand fly species involved in {L}. infantum transmission cycle are still unknown. {B}etween 2007 and 2010, 3654 sand flies were collected from different environments (indoor, peridomestic, farming and sylvatic areas) to identify the main {L}. infantum vector(s). {N}ine sand fly species were identified. {T}he {P}hlebotomus genus (n = 54 specimens; {P}hlebotomus ({P}h) duboscqi and {P}hlebotomus ({P}h). rodhaini) was markedly under-represented in comparison to the {S}ergentomyia genus (n = 3600 specimens; {S}ergentomyia ({S}e) adleri, {S}e. clydei, {S}e. antennata, {S}e. buxtoni, {S}e. dubia, {S}e. schwetzi and {S}e. magna). {S}e. dubia and {S}e. schwetzi were the dominant species indoor and in peridomestic environments, near humans and dogs. {B}lood-meal analysis indicated their anthropophilic behavior. {S}ome {S}e. schwetzi specimens fed also on dogs. {T}he dissection of females in the field allowed isolating {L}. infantum from sand flies of the {S}ergentomyia genus (0.4% of {S}e. dubia and 0.79% of {S}e. schwetzi females). {I}t is worth noting that one {S}e. dubia female not engorged and not gravid revealed highly motile metacyclic of {L}. infantum in the anterior part of the midgut. {PCR}-based diagnosis and sequencing targeting {L}eishmania kinetoplast {DNA} (k{DNA}) highlighted a high rate of {L}. infantum-positive females (5.38% of {S}e. dubia, 4.19% of {S}e. schwetzi and 3.64% of {S}e. magna). {M}ore than 2% of these positive females were unfed, suggesting the parasite survival after blood-meal digestion or egg laying. {L}. infantum prevalence in {S}e. schwetzi was associated with its seroprevalence in dogs and humans and {L}. infantum prevalence in {S}e. dubia was associated with its seroprevalence in humans. {T}hese evidences altogether strongly suggest that species of the {S}ergentomyia genus are probably the vectors of canine leishmaniasis in the {M}ont-{R}olland area and challenge one more time the dogma that in the {O}ld {W}orld, leishmaniasis is exclusively transmitted by species of the {P}hlebotomus genus.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {10},
  numero = {11},
  pages = {e0004940 [17 p.]},
  ISSN = {1935-2735},
  year = {2016},
  DOI = {10.1371/journal.pntd.0004940},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068916},
}