@article{fdi:010068892,
  title = {{L}accase induction by synthetic dyes in {P}ycnoporus sanguineus and their possible use for sugar cane bagasse delignification},
  author = {{H}ernandez, {C}. and {D}a {S}ilva, {A}. {M}. {F}. and {Z}iarelli, {F}. and {P}erraud {G}aime, {I}sabelle and {G}utierrez-{R}ivera, {B}. and {G}arcia-{P}erez, {J}. {A}. and {A}larcon, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he use of synthetic dyes for laccase induction in vivo has been scarcely explored. {W}e characterized the effect of adding different synthetic dyes to liquid cultures of {P}ycnoporus sanguineus on laccase production. {W}e found that carminic acid ({CA}) can induce 722 % and alizarin yellow 317 % more laccase than control does, and they promoted better fungal biomass development in liquid cultures. {A}niline blue and crystal violet did not show such positive effect. {CA} and alizarin yellow were degraded up to 95 % during {P}. sanguineus culturing (12 days). {W}ith this basis, {CA} was selected as the best inducer and used to evaluate the induction of laccase on solid-state fermentation ({SSF}), using sugarcane bagasse ({SCB}) as substrate, in an attempt to reach selective delignification. {W}e found that laccase induction occurred in {SSF}, and a slight inhibition of cellulase production was observed when {CA} was added to the substrate; also, a transformation of {SCB} under {SSF} was followed by the {C}-13 cross polarization magic angle spinning ({CPMAS}) solid-state nuclear magnetic resonance ({NMR}). {R}esults showed that {P}. sanguineus can selectively delignify {SCB}, decreasing aromatic {C} compounds by 32.67 % in 16 days; {O}-alkyl {C} region (polysaccharides) was degraded less than 2 %; delignification values were not correlated with laccase activities. {C}ellulose-crystallinity index was increased by 27.24 % in absence of {CA} and 15.94 % when 0.01 m{M} of {CA} was added to {SCB}; this dye also inhibits the production of fungal biomass in {SSF} (measured as alkyl {C} gain). {W}e conclude that {CA} is a good inducer of laccase in liquid media, and that {P}. sanguineus is a fungus with high potential for biomass delignification.},
  keywords = {{A}lizarin yellow ; {C}arminic acid ; {E}ndoglucanase ; {C}-13 {CPMAS} solid-state ; {NMR} ; {C}ellulose crystallinity ; {MEXIQUE}},
  booktitle = {},
  journal = {{A}pplied {M}icrobiology and {B}iotechnology},
  volume = {101},
  numero = {3},
  pages = {1189--1201},
  ISSN = {0175-7598},
  year = {2017},
  DOI = {10.1007/s00253-016-7890-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068892},
}