@article{fdi:010068885,
  title = {{M}icrobial diversity and cyanobacterial production in {D}ziani {D}zaha crater lake, a unique tropical thalassohaline environment},
  author = {{L}eboulanger, {C}hristophe and {A}gogue, {H}. and {B}ernard, {C}. and {B}ouvy, {M}arc and {C}arr{\'e}, {C}laire and {C}ellamare, {M}. and {D}uval, {C}. and {F}ouilland, {E}. and {G}ot, {P}. and {I}ntertaglia, {L}. and {L}avergne, {C}. and {L}e {F}loc'h, {E}. and {R}oques, {C}. and {S}arazin, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study describes, for the first time, the water chemistry and microbial diversity in {D}ziani {D}zaha, a tropical crater lake located on {M}ayotte {I}sland ({C}omoros archipelago, {W}estern {I}ndian {O}cean). {T}he lake water had a high level of dissolved matter and high alkalinity (10.614.5 g {L}-1 eq. {CO}32-, i.e. 160220 m{M} compare to around 22.5 in seawater), with salinity up to 52 psu, 1.5 higher than seawater. {H}ierarchical clustering discriminated {D}ziani {D}zaha water from other alkaline, saline lakes, highlighting its thalassohaline nature. {T}he phytoplankton biomass was very high, with a total chlorophyll a concentration of 524 to 875 mu g chl a {L}-1 depending on the survey, homogeneously distributed from surface to bottom (4 m). {T}hroughout the whole water column the photosynthetic biomass was dominated (>97% of total biovolume) by the filamentous cyanobacteria {A}rthrospira sp. with a straight morphotype. {I}n situ daily photosynthetic oxygen production ranged from 17.3 to 22.2 g {O}-2 m(-2) d(-1), consistent with experimental production / irradiance measurements and modeling. {H}eterotrophic bacterioplankton was extremely abundant, with cell densities up to 1.5 108 cells m{L}(-1) in the whole water column. {I}solation and culture of 59 {E}ubacteria strains revealed the prevalence of alkaliphilic and halophilic organisms together with taxa unknown to date, based on 16{S} r{RNA} gene analysis. {A} single cloning-sequencing approach using archaeal 16{S} r{DNA} gene primers unveiled the presence of diverse extremophilic {E}uryarchaeota. {T}he water chemistry of {D}ziani {D}zaha {L}ake supports the hypothesis that it was derived from seawater and strongly modified by geological conditions and microbial activities that increased the alkalinity. {D}ziani {D}zaha has a unique consortium of cyanobacteria, phytoplankton, heterotrophic {E}ubacteria and {A}rchaea, with very few unicellular protozoa, that will deserve further deep analysis to unravel its uncommon diversity. {A} single taxon, belonging to the genus {A}rthrospira, was found responsible for almost all photosynthetic primary production.},
  keywords = {{COMORES} ; {MAYOTTE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {12},
  numero = {1},
  pages = {e0168879 [ p.]},
  ISSN = {1932-6203},
  year = {2017},
  DOI = {10.1371/journal.pone.0168879},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068885},
}