@article{fdi:010068803,
  title = {{D}iversity of the bacterial microbiota of {A}nopheles mosquitoes from {B}inh {P}huoc {P}rovince, {V}ietnam},
  author = {{N}go, {C}. {T}. and {R}omano-{B}ertrand, {S}. and {M}anguin, {S}ylvie and {J}umas-{B}ilak, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he naturally acquired microbiota of {A}nopheles can influence vector's susceptibility to {P}lasmodium and its capacity to transmit them. {M}icrobiota modification is a new challenge to limit disease transmission but it still needs advanced knowledges on bacterial community in {A}nopheles, especially in wild and infected specimens from diverse origin and species. {B}acterial culture and 16{S} r{RNA} gene-{PCR} associated to {T}emporal {T}emperature {G}radient {E}lectrophoresis ({TTGE}) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eight {A}nopheles species) collected in the {B}inh {P}huoc {P}rovince, {V}ietnam. {C}ulture and {PCR}-{TTGE} were complementary. {T}he bacterial richness of the mosquito collection encompassed 105 genera belonging to seven phyla, mostly {P}roteobacteria, {F}irmicutes, and {A}ctinobacteria. {S}taphylococcus, {C}lostridium, and {B}acillus in {F}irmicutes were the most prevalent genera. {H}owever, {P}roteobacteria represented by 57 genera was the most diversified phylum in {A}nopheles microbiota. {T}he high overall of {A}nopheles-associated bacteria is confirmed with, to our knowledge, 51 genera described for the first time in {A}nopheles microbiota. {H}owever, the diversity per specimen was low with average diversity index and the average {S}hannon {W}iener score ({H}) of 4.843 and 5.569, respectively. {T}he most represented bacterial genera were present in <30% of the specimens. {C}onsequently, the core microbiota share by {A}nopheles from {B}inh {P}huoc was very narrow, suggesting that {A}nopheles microbiota was greatly influenced by local environments. {T}he repertory of bacterial genera in two specimens of {A}n. dirus and {A}n. pampanai naturally infected by {P}lasmodium vivax was also described as preliminary results. {F}inally, this study completed the repertory of bacteria associated to wild {A}nopheles. {A}nopheles associated-bacteria appeared specimen-dependent rather than mosquitoe species-or group-dependent. {T}heir origin and the existence of {A}nopheles-specific bacterial taxa are discussed.},
  keywords = {{A}nopheles mosquitoes ; abdomen bacterial microbiota ; 16{S} r{RNA} {PCR} - {TTGE} ; malaria ; {B}inh {P}huoc ; {V}ietnam ; {VIET} {NAM}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {7},
  numero = {},
  pages = {art. 2095 [11 ]},
  ISSN = {1664-302{X}},
  year = {2016},
  DOI = {10.3389/fmicb.2016.02095},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068803},
}