@article{fdi:010068796,
  title = {{H}ydrogen production by the hyperthermophilic bacterium {T}hermotoga maritima part {I} : effects of sulfured nutriments, with thiosulfate as model, on hydrogen production and growth},
  author = {{B}oileau, {C}{\'e}line and {A}uria, {R}ichard and {D}avidson, {S}ylvain and {C}asalot, {L}aurence and {C}hristen, {P}ierre and {L}iebgott, {P}ierre-{P}ol and {C}ombet-{B}lanc, {Y}annick},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}hermotoga maritima and {T}. neapolitana are hyperthermophile bacteria chosen by many research teams to produce bio-hydrogen because of their potential to ferment a wide variety of sugars with the highest theoretical {H}-2/glucose yields. {H}owever, to develop economically sustainable bio-processes, the culture medium formulation remained to be optimized. {T}he main aim of this study was to quantify accurately and specifically the effect of thiosulfate, used as sulfured nutriment model, on {T}. maritima growth, yields and productivities of hydrogen. {T}he results were obtained from batch cultures, performed into a bioreactor, carefully controlled, and specifically designed to prevent the back-inhibition by hydrogen. {R}esults: {A}mong sulfured nutriments tested, thiosulfate, cysteine, and sulfide were found to be the most efficient to stimulate {T}. maritima growth and hydrogen production. {I}n particular, under our experimental conditions (glucose 60 mmol {L}-1 and yeast extract 1 g {L}-1), the cellular growth was limited by thiosulfate concentrations lower than 0.06 mmol {L}-1. {U}nder these conditions, the cellular yield on thiosulfate ({Y} {X}/{T}hio) could be determined at 3617 mg mmol(-1). {I}n addition, it has been shown that the limitations of {T}. maritima growth by thiosulfate lead to metabolic stress marked by a significant metabolic shift of glucose towards the production of extracellular polysaccharides ({EPS}). {F}inally, it has been estimated that the presence of thiosulfate in the {T}. maritima culture medium significantly increased the cellular and hydrogen productivities by a factor 6 without detectable sulfide production. {C}onclusions: {T}he stimulant effects of thiosulfate at very low concentrations on {T}. maritima growth have forced us to reconsider its role in this species and more probably also in all thiosulfato-reducer hyperthermophiles. {H}enceforth, thiosulfate should be considered in {T}. maritima as (1) an essential sulfur source for cellular materials when it is present at low concentrations (about 0.3 mmol g(-1) of cells), and (2) as both sulfur source and detoxifying agent for {H}-2 when thiosulfate is present at higher concentrations and, when, simultaneously, the p{H}(2) is high. {F}inally, to improve the hydrogen production in bio-processes using {T}hermotoga species, it should be recommended to incorporate thiosulfate in the culture medium.},
  keywords = {{T}hermotoga maritima ; {H}ydrogen ; {T}hiosulfate ; {P}roductivity ; {G}rowth ; {Y}ields ; {S}ulfured nutriments ; {G}lucose ; {M}etabolism},
  booktitle = {},
  journal = {{B}iotechnology for {B}iofuels},
  volume = {9},
  numero = {},
  pages = {art. 269 [17 p.]},
  ISSN = {1754-6834},
  year = {2016},
  DOI = {10.1186/s13068-016-0678-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068796},
}