@article{fdi:010068778,
  title = {{A}ssay optimization for molecular detection of {Z}ika virus},
  author = {{C}orman, {V}. {M}. and {R}asche, {A}. and {B}aronti, {C}{\'e}cile and {A}ldabbagh, {S}. and {C}adar, {D}. and {R}eusken, {C}bem and {P}as, {S}. {D}. and {G}oorhuis, {A}. and {S}chinkel, {J}. and {M}olenkamp, {R}. and {K}ummerer, {B}. {M}. and {B}leicker, {T}. and {B}runink, {S}. and {E}schbach-{B}ludau, {M}. and {E}is-{H}ubinger, {A}. {M}. and {K}oopmans, {M}. {P}. and {S}chmidt-{C}hanasit, {J}. and {G}robusch, {M}. {P}. and de {L}amballerie, {X}avier and {D}rosten, {C}. and {D}rexler, {J}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective {T}o examine the diagnostic performance of real-time reverse transcription ({RT})-polymerase chain reaction ({PCR}) assays for {Z}ika virus detection. {M}ethods {W}e compared seven published real-time {RT} {PCR} assays and two new assays that we have developed. {T}o determine the analytical sensitivity of each assay, we constructed a synthetic universal control ribonucleic acid (unc{RNA}) containing all of the assays' target regions, on one {RNA} strand and spiked human blood or urine with known quantities of {A}frican or {A}sian {Z}ika virus strains. {V}iral loads in 33 samples from {Z}ika virus-infected patients were determined by using one of the new assays. {F}indings {O}ligonucleotides of the published real-time {RT} {PCR} assays, showed up to 10 potential mismatches with the {A}sian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. {T}he 95% lower detection limit of the seven most sensitive assays ranged from 2.1 to 12.1 unc{RNA} copies/reaction. {T}wo assays had lower sensitivities of 17.0 and 1373.3 unc{RNA} copies/reaction and showed a similar sensitivity when using spiked samples.{T}he mean viral loads in samples from {Z}ika virus-infected patients were 5 x 10(4) {RNA} copies/m{L} of blood and 2 x 10(4) {RNA} copies/m{L} of urine. {C}onclusion {W}e provide reagents and updated protocols for {Z}ika virus detection suitable for the current outbreak strains. {S}ome published assays might be unsuitable for {Z}ika virus detection, due to the limited sensitivity and potential incompatibility with some strains. {V}iral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results.},
  keywords = {},
  booktitle = {},
  journal = {{B}ulletin de l'{O}rganisation {M}ondiale de la {S}ant{\'e}},
  volume = {94},
  numero = {12},
  pages = {880--892},
  ISSN = {0042-9686},
  year = {2016},
  DOI = {10.2471/blt.16.175950},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068778},
}