Publications des scientifiques de l'IRD

Corman V. M., Rasche A., Baronti Cécile, Aldabbagh S., Cadar D., Reusken Cbem, Pas S. D., Goorhuis A., Schinkel J., Molenkamp R., Kummerer B. M., Bleicker T., Brunink S., Eschbach-Bludau M., Eis-Hubinger A. M., Koopmans M. P., Schmidt-Chanasit J., Grobusch M. P., de Lamballerie Xavier, Drosten C., Drexler J. F. (2016). Assay optimization for molecular detection of Zika virus. Bulletin de l'Organisation Mondiale de la Santé, 94 (12), p. 880-892. ISSN 0042-9686.

Titre du document
Assay optimization for molecular detection of Zika virus
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000390083800007
Auteurs
Corman V. M., Rasche A., Baronti Cécile, Aldabbagh S., Cadar D., Reusken Cbem, Pas S. D., Goorhuis A., Schinkel J., Molenkamp R., Kummerer B. M., Bleicker T., Brunink S., Eschbach-Bludau M., Eis-Hubinger A. M., Koopmans M. P., Schmidt-Chanasit J., Grobusch M. P., de Lamballerie Xavier, Drosten C., Drexler J. F.
Source
Bulletin de l'Organisation Mondiale de la Santé, 2016, 94 (12), p. 880-892 ISSN 0042-9686
Objective To examine the diagnostic performance of real-time reverse transcription (RT)-polymerase chain reaction (PCR) assays for Zika virus detection. Methods We compared seven published real-time RT PCR assays and two new assays that we have developed. To determine the analytical sensitivity of each assay, we constructed a synthetic universal control ribonucleic acid (uncRNA) containing all of the assays' target regions, on one RNA strand and spiked human blood or urine with known quantities of African or Asian Zika virus strains. Viral loads in 33 samples from Zika virus-infected patients were determined by using one of the new assays. Findings Oligonucleotides of the published real-time RT PCR assays, showed up to 10 potential mismatches with the Asian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. The 95% lower detection limit of the seven most sensitive assays ranged from 2.1 to 12.1 uncRNA copies/reaction. Two assays had lower sensitivities of 17.0 and 1373.3 uncRNA copies/reaction and showed a similar sensitivity when using spiked samples.The mean viral loads in samples from Zika virus-infected patients were 5 x 10(4) RNA copies/mL of blood and 2 x 10(4) RNA copies/mL of urine. Conclusion We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Some published assays might be unsuitable for Zika virus detection, due to the limited sensitivity and potential incompatibility with some strains. Viral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010068778]
Identifiant IRD
fdi:010068778
Contact