@article{fdi:010068775, title = {{A} single-step method for {RNA} isolation from tropical crops in the field}, author = {{B}reitler, {J}. {C}. and {C}ampa, {C}laudine and {G}eorget, {F}. and {B}ertrand, {B}. and {E}tienne, {H}.}, editor = {}, language = {{ENG}}, abstract = {{T}he {RNA}zol {RT} reagent was used to provide pure {RNA} from human cells. {W}e develop a protocol using {RNA}zol {RT} reagent to extract pure {RNA} from plants tissues and demonstrate that this {RNA} extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded {RNA} directly from tropical plants in the field. {RNA} extraction directly in a complex field environment opens up the way for studying gene-environment interactions at transcriptome level to decipher the complex regulatory network involved in multiple-stress responses.}, keywords = {{ZONE} {TROPICALE}}, booktitle = {}, journal = {{S}cientific {R}eports - {N}ature}, volume = {6}, numero = {}, pages = {art. 38368 [6 p.]}, ISSN = {2045-2322}, year = {2016}, DOI = {10.1038/srep38368}, URL = {https://www.documentation.ird.fr/hor/fdi:010068775}, }