@article{fdi:010068726,
  title = {{C}ulture of previously uncultured members of the human gut microbiota by culturomics},
  author = {{L}agier, {J}. {C}. and {K}helaifia, {S}. and {A}lou, {M}. {T}. and {N}dongo, {S}. and {D}ione, {N}. and {H}ugon, {P}. and {C}aputo, {A}. and {C}adoret, {F}. and {T}raore, {S}. {I}. and {S}eck, {E}. {H}. and {D}ubourg, {G}. and {D}urand, {G}. and {M}ourembou, {G}. and {G}uilhot, {E}. and {T}ogo, {A}. and {B}ellali, {S}. and {B}achar, {D}. and {C}assir, {N}. and {B}ittar, {F}. and {D}elerce, {J}. and {M}ailhe, {M}. and {R}icaboni, {D}. and {B}ilen, {M}. and {N}ieko, {N}pmd and {B}adiane, {N}. {M}. {D}. and {V}alles, {C}. and {M}ouelhi, {D}. and {D}iop, {K}. and {M}illion, {M}. and {M}usso, {D}. and {A}brahao, {J}. and {A}zhar, {E}. {I}. and {B}ibi, {F}. and {Y}asir, {M}. and {D}iallo, {A}. and {S}okhna, {C}heikh and {D}jossou, {F}. and {V}itton, {V}. and {R}obert, {C}. and {R}olain, {J}. {M}. and {L}a {S}cola, {B}. and {F}ournier, {P}. {E}. and {L}evasseur, {A}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{M}etagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism(1). {T}he pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms(2). {W}e recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization-time of flight and 16{S} r{RNA} for identification(2). {H}ere, we have selected the best culture conditions to increase the number of studied samples and have applied new protocols (fresh-sample inoculation; detection of microcolonies and specific cultures of {P}roteobacteria and microaerophilic and halophilic prokaryotes) to address the weaknesses of the previous studies(3-5). {W}e identified 1,057 prokaryotic species, thereby adding 531 species to the human gut repertoire: 146 bacteria known in humans but not in the gut, 187 bacteria and 1 archaea not previously isolated in humans, and 197 potentially new species. {G}enome sequencing was performed on the new species. {B}y comparing the results of the metagenomic and culturomic analyses, we show that the use of culturomics allows the culture of organisms corresponding to sequences previously not assigned. {A}ltogether, culturomics doubles the number of species isolated at least once from the human gut.},
  keywords = {},
  booktitle = {},
  journal = {{N}ature {M}icrobiology},
  volume = {1},
  numero = {12},
  pages = {art. 16203 [8 p.]},
  ISSN = {2058-5276},
  year = {2016},
  DOI = {10.1038/nmicrobiol.2016.203},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068726},
}