@article{fdi:010068679,
  title = {{I}nsight into k13-propeller gene polymorphism and ex vivo {DHA}-response profiles from {C}ameroonian isolates},
  author = {{M}enard, {S}. and {T}choufack, {J}. {N}. and {M}affo, {C}. {N}. and {N}sango, {S}. {E}. and {I}riart, {X}. and {A}bate, {L}uc and {T}sapi, {M}. {T}. and {A}wono-{A}mbene, {P}. {H}. and {M}ekongo, {F}. {A}. {A}. and {M}orlais, {I}sabelle and {B}erry, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he spread of {P}lasmodium falciparum resistance to artemisinin derivatives in {S}outheast {A}sia is a major source of concern and the emergence of resistance in {A}frica would have dramatic consequences, by increasing malaria mortality and morbidity. {I}t is therefore urgent to implement regular monitoring in sentinel sites in sub-{S}aharan {A}frica using robust and easy-to-implement tools. {T}he prevalence of k13-propeller mutations and the phenotypic profiles are poorly known in sub-{S}aharan {A}frica. {H}ere, the k13-propeller polymorphism was compared to both ex vivo susceptibility to {DHA} and early parasitological and clinical responses to artemisinin combination therapy ({ACT}). {M}ethods: {P}lasmodium falciparum isolates were collected in 2015 in {Y}aounde ({C}ameroon) from patients treated with dihydroartemisinin- piperaquine combination. {S}amples were analysed for their susceptibility to artemisinin using the k13-propeller sequencing, the ex vivo ring-stage survival assay, the in vivo parasite positive rate and the clinical statute at day 2. {R}esults: {N}one of the collected isolates revealed the presence of resistance mutations in the k13-propeller sequence. {T}he median ring-stage survival rate for all the 64 interpretable isolates after a 6-hour pulse of 700 n{M} dihydroartemisinin was low, 0.49% ({IQR}: 0-1.3). {T}otal parasite clearance was observed for 87.5% of patients and the remaining parasitaemic isolates (12.5%) showed a high reduction of parasite load, ranging from 97.5 to 99.9%. {C}linical symptoms disappeared in 92.8% of cases. {C}onclusion: {T}his study demonstrated the absence of k13-resistant genotypes in {P}. falciparum isolates from {C}ameroon. {O}nly synonymous mutations were found with a low prevalence (4.3%). {A} good association between k13 genotypes and the ex vivo ring-stage survival assay or parasitological and clinical data was obtained. {T}hese results give a baseline for the long-term monitoring of artemisinin derivative efficacy in {A}frica.},
  keywords = {{A}rtemisinins ; {D}ihydroartemisinin ; {R}esistance ; {P}lasmodium falciparum ; {R}ing-stage survival assay ; {K}13 ; {C}learance ; {C}ameroon ; {CAMEROUN}},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {15},
  numero = {},
  pages = {art. 572 [7 p.]},
  ISSN = {1475-2875},
  year = {2016},
  DOI = {10.1186/s12936-016-1622-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068679},
}