%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Quéméneur, Marianne
%A Garrido, F.
%A Billard, P.
%A Breeze, D.
%A Leyval, C.
%A Jauzein, M.
%A Joulian, C.
%T Bacterial community structure and functional arrA gene diversity associated with arsenic reduction and release in an industrially contaminated soil
%D 2016
%L fdi:010068666
%G ENG
%J Geomicrobiology Journal
%@ 0149-0451
%K arrA genes ; arsenic ; biotransformation ; CE-SSCP ; contaminated soil ; microbial diversity
%K FRANCE ; MEUSE BASSIN
%M ISI:000387909400001
%N 10
%P 839-849
%R 10.1080/01490451.2015.1118167
%U https://www.documentation.ird.fr/hor/fdi:010068666
%> https://www.documentation.ird.fr/intranet/publi/2016/12/010068666.pdf
%V 33
%W Horizon (IRD)
%X This study aimed at evaluating potential arsenic (As) mobility in an industrially contaminated soil (64mg/kg of As) of the Meuse River basin, and at identifying key bacterial groups that drive soil As dynamics. Both speciation and release of As from this soil was followed under anaerobic conditions using a laboratory batch experiment. In the presence of exogenous carbon sources, As-V initially present in the soil matrix and/or adsorbed on synthetic hydrous ferric oxides were solubilized and mainly reduced to As-III by indigenous soil microflora. After a 1-month incubation period in these biotic conditions, As-III accounted for 80-85% of the total dissolved As and more than 60% of the solid-phase As. Bacterial community structure (i.e., 16S rDNA-based capillary electrophoresis single-strand conformation polymorphism profiles) changed with incubation time and As amendment. The detection of distantly related arsenate respiratory reductase genes (arrA), as functional markers of As-V respirers, indicates that novel dissimilatory As-V-reducing bacteria may be involved in As biotransformation and mobility in anoxic soils. Since As and iron were concomitantly released, a crucial role of indirect As-mobilizing bacteria on As behavior was also revealed. Our results show that the majority of As within the soil matrix was bioavailable and bioaccessible for heterotrophic As-V reduction to As-III, which may increase As toxicity and mobility in the contaminated soils.
%$ 038 ; 084 ; 068