%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Moreira, P. A.
%A Mariac, Cédric
%A Scarcelli, Nora
%A Couderc, Marie
%A Rodrigues, D. P.
%A Clément, C. R.
%A Vigouroux, Yves
%T Chloroplast sequence of treegourd (Crescentia cujete, Bignoniaceae) to study phylogeography and domestication
%D 2016
%L fdi:010068304
%G ENG
%J Applications in Plant Sciences
%@ 2168-0450
%K Bignoniaceae ; calabash tree ; Crescentia amazonica ; cuia ; next-generation ; sequencing ; single-nucleotide polymorphism (SNP)
%K BRESIL ; AMAZONIE
%K AMAZONE BASSIN
%M ISI:000386922700001
%N 10
%P 1600048 [4 ]
%R 10.3732/apps.1600048
%U https://www.documentation.ird.fr/hor/fdi:010068304
%> https://www.documentation.ird.fr/intranet/publi/2016/11/010068304.pdf
%V 4
%W Horizon (IRD)
%X Premise of the study: Crescentia cujete (Bignoniaceae) fruit rinds are traditionally used for storage vessels and handicrafts. We assembled its chloroplast genome and identified single-nucleotide polymorphisms (SNPs). Methods and Results: Using a genome skimming approach, the whole chloroplast of C. cujete was assembled using 3,106,928 sequence reads of 150 bp. The chloroplast is 154,662 bp in length, structurally divided into a large single copy region (84,788 bp), a small single copy region (18,299 bp), and two inverted repeat regions (51,575 bp) with 88 genes annotated. By resequencing the whole chloroplast, we identified 66 SNPs in C. cujete (N = 30) and 68 SNPs in C. amazonica (N = 6). Nucleotide diversity was estimated at 1.1 x 10(-3) and 3.5 x 10(-3) for C. cujete and C. amazonica, respectively. Conclusions: This broadened C. cujete genetic toolkit will be important to study the origin, domestication, diversity, and phylogeography of treegourds in the Neotropics.
%$ 076