Rocha D. M., Marques A., Andrade Cgtj, Guyot Romain, Chaluvadi S. R., Pedrosa-Harand A., Houben A., Bennetzen J. L., Vanzela A. L. L. (2016). Developmental programmed cell death during asymmetric microsporogenesis in holocentric species of Rhynchospora (Cyperaceae). Journal of Experimental Botany, 67 (18), p. 5391-5401. ISSN 0022-0957.
Titre du document
Developmental programmed cell death during asymmetric microsporogenesis in holocentric species of Rhynchospora (Cyperaceae)
Année de publication
2016
Auteurs
Rocha D. M., Marques A., Andrade Cgtj, Guyot Romain, Chaluvadi S. R., Pedrosa-Harand A., Houben A., Bennetzen J. L., Vanzela A. L. L.
Source
Journal of Experimental Botany, 2016,
67 (18), p. 5391-5401 ISSN 0022-0957
The functional cell of an asymmetric tetrad is selected according to its replication capability, while degenerative cells progress to multistep PCD, including mitotic disorder and DNA elimination.Members of the Cyperaceae family exhibit an asymmetric microsporogenesis that results in the degeneration of three out of four meiotic products. Efforts have been made previously to describe the resulting structure, named the pseudomonad, but mechanisms concerning the establishment of cell domains, nuclear development, and programmed cell death are largely unknown. Using the Rhynchospora genus as a model, evidence for cell asymmetry, cytoplasmic isolation, and programmed cell death was obtained by a combination of electron microscopic, cytochemical, immunocytochemical, in situ hybridization, and flow cytometric methods. Degenerative cells were identified at the abaxial region, with the cytoskeleton marking their delimitation from the functional domain after meiosis. After attempting to initiate cell division with an unreplicated genome and abnormal spindle assembly, these cells exhibited a gradual process of cytoplasmic contraction associated with hypermethylation of cytosines and differential loss of DNA. These results indicate that the asymmetric tetrad establishes a functional cell, where one nucleus is preferentially selected to survive. Degenerative haploid cells are then eliminated in a multistep process associated with mitotic disorder, non-random elimination of repetitive DNA, vacuolar cell death, and DNA fragmentation.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010068282]
Identifiant IRD
fdi:010068282
